We narrowed to 8,931 results for: Pol

1. pFRT-TO-RPB1-His K1278R CRres si2,4R

   Plasmid

   #139406

   Purpose

   Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K1278R

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiGuide-Neo-NCOA7g1 (BB34)

   Plasmid

   #139452

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

   Plasmid

   #139409

   Purpose

   Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K619, 627, 642R

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

   Plasmid

   #139408

   Purpose

   Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K751, 758, 767R

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFRT-TO-RPB1-His K710,719R CRres si2,4R

   Plasmid

   #139407

   Purpose

   Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K710,719R

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. wtVP1 + VP2C-GFPdeg

   Plasmid

   #166678

   Purpose

   Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFPdeg
   Wild-type Murine polyomavirus VP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. deltaVP1 + VP2C-GFPdeg

    Plasmid

    #166677

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiGuide-Neo-NCOA7g3 (BB36)

    Plasmid

    #139454

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiGuide-Neo-NCOA7g2 (BB35)

    Plasmid

    #139453

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUDP046

    Plasmid

    #107062

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5b HA Irx5 deltaIrot

    Plasmid

    #24995

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of Iro box (aa 314-327), 19 aa upstream …

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCMV5b HA Irx5 deltaC

    Plasmid

    #24996

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletes everything after Hox Domain (aa 112-177).…

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

16. EcM2.1.dtolC

    Bacterial Strain

    #64053

    Purpose

    Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

    Depositor

    George Church

    Article

    Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

    Bacterial Resistance

    Ampicillin

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. EcM2.1 dtolC bla::zeoR

    Bacterial Strain

    #64054

    Purpose

    Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

    Depositor

    George Church

    Article

    Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

    Bacterial Resistance

    Bleocin (zeocin)

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. MG1655 tnaA-

    Bacterial Strain

    #228513

    Purpose

    Indole-responsive Phage Infection Assay using LARP and T7 Phage with gp1 knock-out

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Bacterial Resistance

    None

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2181

    Plasmid

    #158421

    Purpose

    AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple32 (CLDN5 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple32

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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