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We narrowed to 2,355 results for: Superfolder GFP

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Showing: 1521 - 1540 of 2355 results

  1. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

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  2. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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  3. SunTagng14aa

    Plasmid
    #106439
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Publication
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106439-plasmid-map-sequence-id-205526

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  5. Alexandrov Cell-Free Protein Expression Kit (PEK)

    Plasmid kit
    #1000000065
    Purpose
    Plasmids consisting of open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system.
    Depositor
    Kirill Alexandrov
    Application
    Gene Expression and Labeling
    Vector type
    In Vitro Expression
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pSLC-255

    Plasmid
    #124292
    Purpose
    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Publication
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124292-plasmid-map-sequence-id-243084

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  14. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid
    #210021
    Purpose
    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
    Depositor
    Vesa Hytönen
    Publication
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)
    Tags
    MycTag, CTag
    Expression
    Mammalian
    Mutation
    Transferrin receptor transmembrane domain with Y2…
    Promoter
    CMV promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    210021-plasmid-map-sequence-id-425593

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  16. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid
    #137044
    Purpose
    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    T2A-td-sfGFP
    Expression
    Mammalian
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    137044-plasmid-map-sequence-id-274424

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  18. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  19. pYPQ-CBE-SpRYn-Act3.0

    Plasmid
    #178958
    Purpose
    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178958-plasmid-map-sequence-id-354326

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  20. pSLC-284

    Plasmid
    #124294
    Purpose
    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Publication
    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124294-plasmid-map-sequence-id-251247

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Showing: 1521 - 1540 of 2355 results