We narrowed to 7,478 results for: cas9 expression plasmid

1. Tet-inducible Luciferase reporter

   Plasmid

   #64127

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetOx13-CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL4UAS-Luciferase reporter

   Plasmid

   #64125

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   GAL4UAS
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GAL4UAS

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNeurog2-Luciferase reporter

   Plasmid

   #64126

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   Neurog2 promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Neurog2

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS234

   Plasmid

   #73682

   Purpose

   SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

   Plasmid

   #154429

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPRv2_ZsG gRNA_4

   Plasmid

   #192688

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPRv2_ZsG gRNA_1

    Plasmid

    #192685

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2_ZsG gRNA_2

    Plasmid

    #192686

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPRv2_ZsG gRNA_3

    Plasmid

    #192687

    Purpose

    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

    Depositor

    Jeffrey Miner

    Insert

    Cas9, ZsGreen targeting sgRNA #3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA1_pNeurog2-Luciferase reporter

    Plasmid

    #64160

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for pNeurog2-Luciferase reporter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZAK gRNA (BRDN0001145178)

    Plasmid

    #75596

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAK
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ZAK gRNA (BRDN0001146540)

    Plasmid

    #75597

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAK
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SGK223 gRNA (BRDN0001146707)

    Plasmid

    #75791

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGK223

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SGK223
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SGK223 gRNA (BRDN0001147337)

    Plasmid

    #75792

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGK223

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SGK223
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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