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pUG34-NES-EGFP-cPHx3

Plasmid

#183669

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.L858R

Plasmid

#82906

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

L858R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hDNAJC6-T2A-copGFP

Plasmid

#170443

Purpose

Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human DNAJC6 (DNAJC6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPP1_WT_V5

Plasmid

#82981

Purpose

Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SPP1 (SPP1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SHC1_WT_V5

Plasmid

#82931

Purpose

Gateway Donor vector containing SHC1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SHC1 (SHC1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CBR1_WT_V5

Plasmid

#82945

Purpose

Gateway Donor vector containing CBR1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CBR1 (CBR1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPos-Ma-CmR-TAG111

Plasmid

#197571

Purpose

Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

Chloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)

Expression

Bacterial

Mutation

111TAG and 8TAG/112TAG

Promoter

AraC, T7, cat promoter (constitutive), and lpp (c…

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-LAMP1-HaloTag

Plasmid

#164216

Purpose

pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

HaloTag

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E545K

Plasmid

#82881

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway entry vector

Mutation

E545K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAVCR2_WT

Plasmid

#82893

Purpose

Gateway Donor vector containing HAVCR2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

HAVCR2 (HAVCR2 Human)

Use

Gateway entry vector

Mutation

R140L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TP53_WT

Plasmid

#82754

Purpose

Gateway Donor vector containing TP53, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TP53 (TP53 Human)

Use

Gateway entry vector

Mutation

P72R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

Plasmid

#159697

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.T790M_p.L858R

Plasmid

#82914

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

T790M, L858R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G469V

Plasmid

#82833

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G469V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.R470S

Plasmid

#82781

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

R470S; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Human 3' HP1a AID GFP PuroR

Plasmid

#127906

Purpose

HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

HP1 (CBX5 Human, Mustard Weed)

Use

Donor plasmid

Tags

GFP, AID, PuroR

Mutation

Inserting GFP AID 2A Puro into mouse 3' Hp1a

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_WT_V5

Plasmid

#83004

Purpose

Gateway Donor vector containing ZBTB24, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_WT

Plasmid

#82888

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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