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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,128 results for: try

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Showing: 1521 - 1540 of 3128 results

  3. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  4. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113687
    Purpose
    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113687-plasmid-map-sequence-id-231825

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  7. DHRS4

    Plasmid
    #39071
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DHRS4 (DHRS4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pENTR-AtTAS1c-D2-B/c

    Plasmid
    #137883
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Publication
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Gateway-compatible entry clone
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    137883-plasmid-map-sequence-id-275808

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  10. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Depositor
    Henry Lester, Jerry Stitzel
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    48811-plasmid-map-sequence-id-193963

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  12. Epac-S-H171

    Plasmid
    #170353
    Purpose
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170353-plasmid-map-sequence-id-345820

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  14. pPK-351

    Plasmid
    #157921
    Purpose
    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD
    Depositor
    Phillip Kyriakakis
    Publication
    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    Use
    Synthetic Biology; Optogenetics
    Tags
    HA tag and SV40NLS
    Expression
    Mammalian
    Mutation
    pif3 1-523
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157921-plasmid-map-sequence-id-311915

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  19. pKCAG-mCherry-OptoJNKi

    Plasmid
    #89738
    Purpose
    Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OptoJNKi
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89738-plasmid-map-sequence-id-178312

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  20. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

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Showing: 1521 - 1540 of 3128 results