164,728 results

1. pYPQAT42

   Plasmid

   #223414

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQAT21

   Plasmid

   #223393

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQAT09

   Plasmid

   #223381

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMRXIP-GFP-Evectin-2

   Plasmid

   #221754

   Purpose

   Stable expression of Evectin-2 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PLEKHB2 (PLEKHB2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   tandem PLEKHB2(PH domain) (amino acids 1-110)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-CMV-PE2-IRES-ZeoR

   Plasmid

   #207352

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor 2 (PE2) enzyme
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

   Plasmid

   #207356

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Anti-TrpC4 [N77/15.1R]

   Plasmid

   #114504

   Purpose

   Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. B-Sniper SpCas9

   Plasmid

   #207361

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTXBI-mClover-YTH domain

   Plasmid

   #177123

   Purpose

   Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH domain) (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   YTH domain only (IDR1 and IDR2 deletion)

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-ataxin-3 *VBM

    Plasmid

    #89976

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 282RKRR to 282HNHH

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge