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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,483 results for: NSI

Showing: 3081 - 3120 of 12483 results

  1. pAL204

    Plasmid
    #22282
    Purpose
    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2010
    Availability
    Academic Institutions and Nonprofits only
    22282-plasmid-map-sequence-id-80604

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  2. LRP6 dC-pCS2

    Plasmid
    #27258
    Depositor
    Xi He
    Article
    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.
    Insert
    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    Use
    Xenopus injection
    Tags
    Expression
    Mammalian
    Mutation
    carboxyl intracellular region deleted Residue 14…
    Promoter
    Available since
    Feb. 10, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Article
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  7. mCit-Snap25b D179K A199R

    Plasmid
    #53259
    Purpose
    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Use
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    D179K and A199R
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. CMV-R-GECO1

    Plasmid
    #32444
    Purpose
    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    R-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32444-plasmid-map-sequence-id-117093

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  14. CMV-NLS-R-GECO

    Plasmid
    #32462
    Purpose
    Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    R-GECO1.0
    Use
    Tags
    NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32462-plasmid-map-sequence-id-201367

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  15. pAL113

    Plasmid
    #22471
    Purpose
    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    PhyB NT (PHYB Mustard Weed, Mouse)
    Use
    Synthetic Biology
    Tags
    10aaLinker-mCFP-HrasCT
    Expression
    Mammalian
    Mutation
    Y276H
    Promoter
    CMV
    Available since
    Nov. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    22471-plasmid-map-sequence-id-10325

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  26. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  27. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

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  29. pTT5-hSDC1-CS2

    Plasmid
    #52367
    Purpose
    expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R95Q and S194A (according to numbering in Fig. 2A…
    Promoter
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. Nemato

    Plasmid
    #24006
    Depositor
    Lawrence Cohen
    Insert
    VSFP
    Use
    Tags
    Cerulean and Citruline
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2010
    Availability
    Academic Institutions and Nonprofits only
    24006-plasmid-map-sequence-id-11277

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  31. pcDNA-FLAG-Nv-IKK

    Plasmid
    #27262
    Depositor
    Thomas Gilmore
    Insert
    Nv-IKK
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  32. pcDNA-FLAG-Nv-IkB

    Plasmid
    #27261
    Depositor
    Thomas Gilmore
    Insert
    Nv-IkB
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    P88Q
    Promoter
    Available since
    Feb. 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  33. pcDNA-FLAG-Nv-Bcl-3

    Plasmid
    #27263
    Depositor
    Thomas Gilmore
    Insert
    Nv-Bcl-3
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  34. pTS_ABS

    Plasmid
    #163840
    Purpose
    iPTG inducible expression of abietadiene synthase and geranylgeranyl diphosphate synthase
    Depositor
    Jerome Fox
    Insert
    abietadiene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Trc
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163840-plasmid-map-sequence-id-339140

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  36. pGreenFire1_E2F1RE

    Plasmid
    #112248
    Purpose
    expression of GFP and Luciferase under the control of E2F1 responsive element
    Depositor
    Simone Di Giovanni
    Insert
    E2F1RE (responsive element)
    Use
    Lentiviral
    Tags
    copGFP and luciferase
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112248-plasmid-map-sequence-id-213659

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  37. pTS_TXS

    Plasmid
    #163839
    Purpose
    iPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthase
    Depositor
    Jerome Fox
    Insert
    taxadiene synthase (61-862)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Trc
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163839-plasmid-map-sequence-id-335623

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  38. pJBL7058

    Plasmid
    #167227
    Purpose
    overexpression of orthogonal T7 RNAP
    Depositor
    Julius Lucks
    Insert
    J23119-AKSIRV
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167227-plasmid-map-sequence-id-332706

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  39. pGL1_108

    Plasmid
    #210692
    Purpose
    Level 1 plasmid, barcoded ORI
    Depositor
    Cultivarium Tools, Nili Ostrov, Charlie Gilbert
    Insert
    pIM13 with barcode 28
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210692-plasmid-map-sequence-id-417382

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  40. pJBL7055

    Plasmid
    #167224
    Purpose
    orthogonal T7 RNAP sfGFP reporter
    Depositor
    Julius Lucks
    Insert
    pRV-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167224-plasmid-map-sequence-id-331030

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Showing: 3081 - 3120 of 12483 results