We narrowed to 25,030 results for: Nes

1. pJAK184.tetR.PT5-3.dCas9

   Plasmid

   #232482

   Purpose

   optimized ahTet inducible dCas9 for insertion into C. difficile genome

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Insert

   Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-PT5-3

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFR-mEGFP

   Plasmid

   #203774

   Purpose

   Number and brightness analysis of EGFR oligomerization

   Depositor

   Thorsten Wohland

   Article

   Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p16 nlsGPS2

   Plasmid

   #228093

   Purpose

   Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

   Depositor

   Alexander Jones

   Article

   Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

   Insert

   nlsGPS2
   

   Use

   Tags

   6xHis-T7 XpressTag and cMyc

   Expression

   Plant

   Mutation

   Promoter

   p16

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXL-BacII-CAG-loxP-STOP-loxP-Venus

   Plasmid

   #170520

   Purpose

   Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

   Insert

   CAG-loxP-STOP-loxP-Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. P447-FBP_19

   Plasmid

   #139710

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR-v2-Blast-Puro

   Plasmid

   #167186

   Purpose

   Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT10R

   Plasmid

   #98307

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERG20 N127W

   Promoter

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT2

    Plasmid

    #98306

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 N127W

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYL156 (TRV RNA2)

    Plasmid

    #148969

    Purpose

    Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    Modified TRV RNA2
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    see comments section below

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe HA-Runx1-neo

    Plasmid

    #45815

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. shCTL-mlpx-puro

    Plasmid

    #65232

    Purpose

    encodes a non-targeting control shRNA

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    non-coding shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. shCTL-mlpx-neo

    Plasmid

    #65233

    Purpose

    encodes a non-targeting control shRNA

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    non-coding shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Tags

    Expression

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid

    #194328

    Purpose

    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

    Depositor

    Alexander McLellan

    Article

    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Tet-responsive promotor related mutations: G72V i…

    Promoter

    Tight tet-responsive promotor

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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