We narrowed to 10,970 results for: T7
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Plasmid#124252PurposeIn vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templatePromoterT7Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV129
Plasmid#124253PurposeIn vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templatePromoterT7Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBB0405-TEV-His12
Plasmid#137040Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66795.1 (BB_0405 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRevA-TEV-His12
Plasmid#137034Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12DepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pP13-TEV-His12
Plasmid#137035Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd96)
Plasmid#177901PurposeBacterial expression of his10-tagged human cd96 Intracellular DomainDepositorInsertCD96 (aa 557-585 only) (CD96 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A T7RNAP
Bacterial Strain#182776PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically insertedDepositorBacterial ResistanceSpectinomycinAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVTRup
Plasmid#101362PurposeHighly efficient in vitro transcription. Empty backbone.DepositorInsert5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
UseFor cloning a gene for in vitro transcription pur…PromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(1-611)
Plasmid#39336DepositorInsertElongation factor 1 alpha-like protein
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7
Plasmid#226197Purposebacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purificationDepositorInsertHaloTag.v7
Tags6xHISExpressionBacterialPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5Y33
Plasmid#234204PurposeHeterologous protein expression of alginate lyase in Escherichia coliDepositorInsert5Y33
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-T7EI
Plasmid#165540PurposeDNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).DepositorInsertT7EI (T7 Endonuclease I)
UseSynthetic BiologyAvailable SinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneTagsGST (glutathione S-transferase)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p28BIOH-LIC
Plasmid#62352PurposeE. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tagDepositorTypeEmpty backboneTags6xHis and AviTagExpressionBacterialPromoterT7-lacOAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1gamma
Plasmid#51770PurposeExpresses PP1 gamma residues 7-323DepositorInsertpp1 gamma
TagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 gamma residues 7-323PromoterT7-lacAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1beta
Plasmid#51769PurposeExpresses PP1 beta residues 6-327DepositorInsertpp1 beta
TagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 beta residues 6-327PromoterT7-lacAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PRKCBP1A-c092
Plasmid#53633Purposebacterial expression plasmidDepositorInsertPRKCBP1A
ExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
3C8X
Plasmid#51336DepositorInsert3C8X
UseBaculovirus expressionTagsHisMutationTruncation: See CommentsPromoterT7Available SinceFeb. 24, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits