We narrowed to 12,857 results for: sequence
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Plasmid#53429PurposeExpresses full-length Vitronectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertVTN (VTN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUbC-nls-ha-stdMCP-stdGFP
Plasmid#98916Purposesynonymized tandem dimer MCP fused to synonymized tandem dimer GFPDepositorInsertstdMCP-stdGFP
UseLentiviralTagsGFPExpressionMutationPromoterUbCAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab7
Plasmid#174457PurposeExpress EGFP with late endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with late endosome-targeting sequence
UseTagssmall GTPase (Rab7)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-EEA1
Plasmid#174451PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
UseTagsearly endosomal antigen 1 (EEA1)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab5
Plasmid#174454PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
UseTagssmall GTPase (Rab5)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
UseTagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17FNV
Plasmid#118838PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17FNV (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationFNV: L46F, V53N, E57VPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-KRasCT
Plasmid#175835PurposeExpress mCherry with plasma membrane-targeting sequence in mammalian cellsDepositorInsertmCherry with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-mCherry-CDMPR
Plasmid#182643PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)DepositorInsertCDMPR
UseRetroviralTagsN-terminal ER targeting sequence of hemagglutinin…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-EGFP-CDMPR
Plasmid#182642PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)DepositorInsertCDMPR
UseRetroviralTagsEGFP and N-terminal ER targeting sequence of hema…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTS-HA-GFP11
Plasmid#177725PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA taggedDepositorInsertMTS-HA-GFP11
UseTagsGFP11 and HAExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCL_P2A_Hygro_Barcode
Plasmid#120462PurposeBarcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)
Plasmid#193048PurposeHeatshock expressed Cas9DepositorInserthsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
UseCRISPRTagsExpressionWormMutationPromoterhsp16.41pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_TBX5_P2A_Hygro_Barcode
Plasmid#120486PurposeBarcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB
Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only