Addgene

We narrowed to 3,333 results for: Ubc

Showing: 1561 - 1580 of 3333 results

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pcDNA3.1(+)mGAT1YFP3

Plasmid

#41667

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 3 C-terminal-m…

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pC1-IMS-oROS-HT

Plasmid

#216419

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Shank [N23B/49R]

Plasmid

#128637

Purpose

Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Shank Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-4* [N98/7R]

Plasmid

#140081

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-4* (Mouse). Derived from hybridoma N98/7.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-4* (Mouse) recombinant mouse monoclonal antibody (Nlgn4l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-His

Plasmid

#67866

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP3 (KIAA0391 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Ankyrin [N388A/60R]

Plasmid

#128640

Purpose

Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SVOP [N356/23R]

Plasmid

#114499

Purpose

Mammalian Expression Plasmid of anti-SVOP (Rat). Derived from hybridoma N356/23.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SVOP (Rattus norvegicus) recombinant mouse monoclonal antibody (Svop Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SCG10/Stathmin-2 [L5/1R]

Plasmid

#140065

Purpose

Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-nAChr-beta3

Plasmid

#48811

Purpose

This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

Depositor

Henry Lester, Jerry Stitzel

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-FHF-A [N235/22R]

Plasmid

#114509

Purpose

Mammalian Expression Plasmid of anti-Pan-FHF-A (Human). Derived from hybridoma N235/22.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-FHF-A (Homo sapiens) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Pannexin-2 [N121A/31R]

Plasmid

#140073

Purpose

Mammalian Expression Plasmid of anti-Pannexin-2 (Rat). Derived from hybridoma N121A/31.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pannexin-2 (Rat) recombinant mouse monoclonal antibody (Panx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX-TIR-GFP

Plasmid

#52737

Purpose

Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

Depositor

Jonathan Kagan

Article

Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

Insert

Toll/IL-1 Receptor adaptor protein (TIRAP Human)

Use

Retroviral

Tags

GFP and p40 Phox lipid binding domain

Expression

Mammalian

Mutation

Deleted amino acids 1-85

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK7-EE-NLS hygro

Plasmid

#87780

Purpose

Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7 (Map2k7 Mouse)

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyso-ExRai-CKAR2

Plasmid

#236100

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

LAMP1-ExRai-CKAR2 (LAMP1 Synthetic, Human)

Use

Tags

Full-length LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBX_Sec61b_smGFP_Myc

Plasmid

#236092

Purpose

Constitutively encodes Sec61b-smGFP-Myc (sfGFP-based spaghetti monster fluorescent protein embedding Myc epitope tags), for targeting the endoplasmic reticulum.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Sec61b-smGFP-Myc (SEC61B Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBX_RPS8_smRuby2_HA

Plasmid

#236089

Purpose

Constitutively encodes RPS8-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting RPS8 in the 40S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPS8-smRuby2-HA (RPS8 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT_LF(C199S)

Plasmid

#216412

Purpose

Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT_LF(C199S)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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