We narrowed to 4,198 results for: erf

1. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

   Plasmid

   #149595

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10TC), Psyn

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

   Plasmid

   #149591

   Purpose

   all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10TC), Psyn

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S(-10TC)_Psyn-sgRNArodA

   Plasmid

   #149657

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10TC), Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(-10TC)_Psyn-sgRNAflaB

   Plasmid

   #149589

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10TC), Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBz172_CsoS2_N1_GFP

   Plasmid

   #140852

   Purpose

   His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 1 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBz175_CsoS2_N4_GFP

   Plasmid

   #140855

   Purpose

   His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 4 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBz173_CsoS2_N2_GFP

   Plasmid

   #140853

   Purpose

   His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 2 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBz174_CsoS2_N3_GFP

   Plasmid

   #140854

   Purpose

   His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 3 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLz26_CsoS2_N1-N2_GFP

   Plasmid

   #140851

   Purpose

   His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeats 1-2 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-PTEN-3'UTR

    Plasmid

    #97204

    Purpose

    Expresses PTEN 3'UTR in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

    Insert

    PTEN 3' UTR (PTEN Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPOR2KN0077

    Plasmid

    #117644

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0099)and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0110)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PDZK1_PDZ_2

    Plasmid

    #103943

    Purpose

    Protein expression and purification of PDZK1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDZK1_PDZ_3

    Plasmid

    #103944

    Purpose

    Protein expression and purification of PDZK1 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBV163

    Plasmid

    #108364

    Purpose

    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    

    Use

    Replace c.glabrata yap1 promoter with c. glabrata…

    Tags

    Expression

    Mutation

    Promoter

    TDH3

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas59

    Plasmid

    #82396

    Purpose

    sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas57

    Plasmid

    #82395

    Purpose

    sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas56

    Plasmid

    #82394

    Purpose

    sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas55

    Plasmid

    #82393

    Purpose

    sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCas52

    Plasmid

    #82392

    Purpose

    sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB425

    Plasmid

    #74819

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge