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pEMB018 / LRRK2_BC_1867_2527_M2397T

Plasmid

#40537

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

aa1867_2527; M2397T

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1882_2527_M2397T

Plasmid

#40538

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

aa1882_2527; M2397T

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB52 LRRK2_BC_1520_2210_I2211ENLYFQGWSHPQFEK

Plasmid

#40565

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and Tev-Strep (ENLYFQGWSHPQFEK)

Mutation

WT; aa1520_2210

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB52 LRRK2_BC_1520_2148_I2149ENLYFQGWSHPQFEK

Plasmid

#40566

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and Tev-Strep (ENLYFQGWSHPQFEK)

Mutation

WT; aa1520_2148

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 LRRK2_BC_1520_2148_K1906M

Plasmid

#40567

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

aa1520_2148; K1906M

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMB017 NP_9409803_LRRK2_1867_2161_L1914N_V2048T_F2083Y

Plasmid

#40427

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

aa1867_2161; L1914N, V2048T, F2083Y

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1300_2148

Plasmid

#40500

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1300_2148

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMB018 / LRRK2_BC_1300_2170

Plasmid

#40501

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa300_2170

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB018 / LRRK2_BC_1300_2210

Plasmid

#40502

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1300_2210

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

Plasmid

#139015

Purpose

To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

HA-MAO

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Assembled_PUTRA_PURO_HA-RHOA-T19N

Plasmid

#252259

Purpose

Express HA-RHOA(T19N)

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

RHOA T19N

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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Assembled_PUTRA_PURO_HA-RHOA-Y42C

Plasmid

#252261

Purpose

Express HA-RHOA(Y42C)

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

RHOA Y42C

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-EGFP-pA

Plasmid

#200520

Purpose

Mini circle middle entry plasmid for membrane GFP- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

memb-GFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-mCherry-pA

Plasmid

#200528

Purpose

mini circle middle entry plasmid for membrane mCherry-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-BFP-pA

Plasmid

#200537

Purpose

mini circle middle entry plasmid for membrane BFP-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-Membrane

Plasmid

#168832

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-Membrane (M )

Use

Gateway Cloning

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-229E-Membrane

Plasmid

#168966

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-NL63-Membrane

Plasmid

#168925

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Membrane (HCNV63gp5 )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Membrane

Plasmid

#168946

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-HKU1-Membrane

Plasmid

#168905

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-2-Membrane

Plasmid

#168880

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_SARS-CoV-1-Membrane

Plasmid

#168840

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-Membrane (M )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa-C3

Plasmid

#185587

Purpose

to recruit Vasa-C3 to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-C3

Use

In vitro transcription (mrna synthesis)

Tags

mCherry and membrane targeting sequence from Neur…

Mutation

C3 mutant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa

Plasmid

#185586

Purpose

to recruit Vasa to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry, S-Tag and membrane targeting sequence fr…

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Mutation

K82R kinase-dead mutant

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

Pooled Library

#83994

Purpose

Mouse CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
cASIC1 concatemer - fully assembled

Plasmid

#233133

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway Cloning

Tags

mOrange2

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163063

Purpose

Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Gateway Cloning

Tags

eGFP

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)

Plasmid

#160581

Purpose

Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminator

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Human SLC KO Library

Pooled Library

#132552

Purpose

Knockout library targeting human SLC proteins.

Depositor

Giulio Superti-Furga

Article

Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
2C-3XtbGFP-PEST

Plasmid

#69072

Purpose

Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

turboGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Article

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
K44A dynamin 1 pEGFP

Plasmid

#34681

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pVT0494 MULTI-assembly vector with HuABC2 Variable Heavy Domain

Plasmid

#252076

Purpose

Vector for MULTI-assembly cloning for HuABC2 Variable Heavy Domain variants; replace HuABC2 insert with gene of interest via Gibson Assembly

Depositor

Patrick Hsu

Article

Tran et al Science. 2026 Feb 19:eaea1820. doi: 10.1

Insert

HuABC2 variable heavy domain

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-EGFP-ssHRP-TM

Plasmid

#31156

Depositor

Hollis Cline

Article

Li et al Front Neural Circuits. 2010 Feb 26;4:6.

Insert

ssHRP-TM

Use

Membrane targeted hrp (mhrp)

Expression

Mammalian

Mutation

includes signal sequence and a transmembrane doma…

Promoter

CMV promoter including immediate early promoter/e…

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
wt dynamin 1 pEGFP

Plasmid

#34680

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5B-Flag-Smurf1 C699A

Plasmid

#11753

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

C699A: catalytically inactive

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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