We narrowed to 157 results for: AsCas12a

1. Humagne Set C

   Pooled Library

   #172650

   Purpose

   Set C contains best-ranked guides 1 and 4.

   Depositor

   David Root, John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Expression

   Mammalian

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

2. Humagne Set D

   Pooled Library

   #172651

   Purpose

   Set D contains best-ranked guides 2 and 3.

   Depositor

   David Root, John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Expression

   Mammalian

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

3. enAsABE8e

   Plasmid

   #138506

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-dAsCas12a (En)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRDA_887

   Plasmid

   #201164

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDA_888

   Plasmid

   #201165

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. enAsABE8e-dimer

   Plasmid

   #138507

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ223 (dAsCpf1-SRDX)

   Plasmid

   #86209

   Purpose

   Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   dAsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   NLS and SRDX repressor and NLS

   Expression

   Plant

   Mutation

   D908A

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDA_052_HygroR

    Plasmid

    #228937

    Purpose

    Plasmid for cloning of custom gRNA using EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

    Plasmid

    #199605

    Purpose

    Expression of cell permeable opAsCas12a in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    opCas12a-T8N
    

    Use

    CRISPR

    Tags

    6xHis, Twin-strep, SUMO

    Expression

    Bacterial

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. WN10150

    Plasmid

    #80443

    Purpose

    Expresses dead (908A) AsCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    nuclease dead (908A) AsCpf1
    

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    nuclease dead (D908A)

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Inzolia library in pRDA_550

    Pooled Library

    #209552

    Purpose

    The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

17. Inzolia library in pRDA_052

    Pooled Library

    #209551

    Purpose

    The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only