We narrowed to 12,552 results for: NUC

1. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-Nuc-ExRai-AMPKAR

   Plasmid

   #192450

   Purpose

   ExRai-AMPKAR targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD434-SR mouse Alpha-synuclein

   Plasmid

   #89073

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. V5-BS2-SENP2 (Nuclear Pore)

   Plasmid

   #220345

   Purpose

   Mammalian expression vector BS2 in nuclear pore

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   SENP2 (nuclear pore localized protein) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH-nuc-roGFP2-ORP1

   Plasmid

   #191758

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nuc-mTurq2-Apollo-NADP+

   Plasmid

   #193302

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   SV40 NLS and mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH-nuc-roGFP2-GPXC71S

   Plasmid

   #191760

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-GPXC71S
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   GPX has no peroxidatic Cysteine 71, which is repl…

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.0_nucLAMA-F98 IRES EGFP

   Plasmid

   #136618

   Purpose

   Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV NucleoBAS N354K-I372V

    Plasmid

    #63574

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS N354K-I372V
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    N345K and I372V

    Promoter

    CMV

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pOpen-Endonuclease VIII E. coli

    Plasmid

    #165561

    Purpose

    Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Endonuclease VIII E. coli
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid

    #52478

    Purpose

    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. nuc-yHS1-M7A,H102A

    Plasmid

    #159168

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A,H102A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCDNA3.0_nucLAMA-G97 IRES mYFP

    Plasmid

    #130713

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.0_nucLAMA-F98 IRES mYFP

    Plasmid

    #130712

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColdII-SIRPT1-Nelumbo nucifera

    Plasmid

    #75358

    Purpose

    Nt His-tag Nelumbo nucifera Sacsin 1-1313

    Depositor

    Kalle Gehring

    Insert

    Sacsin Nelumbo nucifera 1-1313
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRVdG-mCherry-N-P-M-nucBFP-EGFP-L

    Plasmid

    #52484

    Purpose

    Expresses mCherry, nuclear mTagBFP and EGFP

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    mCherry
    nuclear mTagBFP
    EGFP
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pD434-SR rat Alpha-synuclein

    Plasmid

    #89076

    Purpose

    bacterial expression of rat Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

    Plasmid

    #194175

    Purpose

    Expresses lncRNA HIF1a-AS1 mutant

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    HIF1a-AS1 (HIF1A-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Within Exon1, nucleotides 26-78 were removed

    Promoter

    CMV

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-SIRPT1-Nelumbo nucifera

    Plasmid

    #75326

    Purpose

    GST fusion Nelumbo nucifera Sacsin 1-1313

    Depositor

    Kalle Gehring

    Insert

    Sacsin Nelumbo nucifera 1-1313
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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