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BsSrfD

Plasmid

#242095

Purpose

Thioesterase from Bacillus subtilis

Depositor

Audrey Lamb

Article

Ronnebaum et al Biochemistry. 2025 Sep 16;64(18):3919-39

Insert

BsSrfD

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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SM470_EF1A-dEnAsCas12a-T2A-EGFP

Plasmid

#244222

Purpose

Mammalian expression of dEnAsCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

Plasmid

#244212

Purpose

Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFP

Plasmid

#244214

Purpose

Mammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVB002

Plasmid

#127186

Purpose

Tunable NOT gate with RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate with RiboJ and Reporter

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight 2.4

Plasmid

#37402

Purpose

Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight 2.4

Tags

ZsGreen1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

Plasmid

#244204

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

Plasmid

#244219

Purpose

Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTKDP-neo

Plasmid

#71320

Purpose

Donor plasmid - kanamycin resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTKDP-dhfr

Plasmid

#71323

Purpose

Donor plasmid - trimethoprim resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcSrc527

Plasmid

#17675

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Tyr 527 changed to Phe . Activates transforming a…

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTKDP-cat

Plasmid

#71321

Purpose

Donor plasmid - chloramphenicol resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMLC-BB1

Plasmid

#166922

Purpose

Backbone for assembly of multi-level gene expression controllers

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

CyOFP - Orange fluorescent protein - flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100 constitutive

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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