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pVB002

Plasmid

#127186

Purpose

Tunable NOT gate with RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate with RiboJ and Reporter

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pREMORAv1

Plasmid

#248172

Purpose

pSC101-ts plasmid with salicylic acid inducible Lambda-RED operon and constituitive recA expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaRED

Expression

Bacterial

Mutation

None

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pSHARK2

Plasmid

#248168

Purpose

pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-B0033-PirWT) expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaCI-PirWT

Expression

Bacterial

Mutation

None

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight 2.4

Plasmid

#37402

Purpose

Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight 2.4

Tags

ZsGreen1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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SM470_EF1A-dEnAsCas12a-T2A-EGFP

Plasmid

#244222

Purpose

Mammalian expression of dEnAsCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTKDP-neo

Plasmid

#71320

Purpose

Donor plasmid - kanamycin resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTKDP-dhfr

Plasmid

#71323

Purpose

Donor plasmid - trimethoprim resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcSrc527

Plasmid

#17675

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Tyr 527 changed to Phe . Activates transforming a…

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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pET22b_pScaC-SNAP_6xHis

Plasmid

#228835

Purpose

E. coli expression of the SNAP-tagged ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

SNAPtag-TEV-6xHis

Expression

Bacterial

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTKDP-cat

Plasmid

#71321

Purpose

Donor plasmid - chloramphenicol resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMLC-BB1

Plasmid

#166922

Purpose

Backbone for assembly of multi-level gene expression controllers

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

CyOFP - Orange fluorescent protein - flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100 constitutive

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTHS

Plasmid

#166938

Purpose

Toehold switch multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Toehold switch

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTKDP-hph

Plasmid

#71322

Purpose

Donor plasmid - hygromycin resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcSrc416

Plasmid

#17676

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src Y416F (SRC Chicken)

Expression

Mammalian

Mutation

Tyr 416 changed to Phe .

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pPtac

Plasmid

#166937

Purpose

Ptac single-level gene expression controller (SLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- PTAC

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDC

Plasmid

#166941

Purpose

Dual control based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Dual Control

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pH-01

Plasmid

#166936

Purpose

Reverse Ptac promoter part (H)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

PTAC promoter - Block H- Flanked by BsaI restriction sites.

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-01

Plasmid

#166932

Purpose

Toehold switch part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Toehold Switch - Block G - Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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