We narrowed to 1,594 results for: PTS

1. AP2u2-CMV-Ace2-IgG1

   Plasmid

   #226455

   Purpose

   Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

   Inserts

   Angiotensin Converting Enzyme 2
   Immunoglobulin Heavy Constant Gamma 1
   

   Use

   Tags

   6xHis Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-PEX7 [N232/9R]

   Plasmid

   #206544

   Purpose

   Mammalian Expression Plasmid of anti-PEX7 (Human). Derived from hybridoma N232/9.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-PEX7 (Homo sapiens) recombinant Mouse monoclonal antibody (PEX7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX-53BP1(1-1710) 3LA

   Plasmid

   #185698

   Purpose

   Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.

   Depositor

   Daniel Durocher

   Article

   Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

   Insert

   53BP1(1-1710) 3LA (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   Flag tag, HA tag, and tetracysteine tag

   Expression

   Mammalian

   Mutation

   L175A, L517A, and L693A

   Promoter

   SV40

   Available since

   July 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMX-53BP1(1-1710) 3LA7A

   Plasmid

   #185707

   Purpose

   Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.

   Depositor

   Daniel Durocher

   Article

   Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

   Insert

   53BP1(1-1710) 3LA7A (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   Flag tag, HA tag, and tetracysteine tag

   Expression

   Mammalian

   Mutation

   T302A, S452A, S523A, S543A, S625A, S784A, and S89…

   Promoter

   SV40

   Available since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

   Plasmid

   #223777

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)

   Plasmid

   #223779

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   ATG13 IDR (ATG13 Human)
   

   Use

   Tags

   GST-EGFP

   Expression

   Bacterial

   Mutation

   expresses aa 191-517, with M196A S197A R199A G202…

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_DNTTIP2_WT_V5

   Plasmid

   #82939

   Purpose

   Gateway Donor vector containing DNTTIP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   DNTTIP2 (DNTTIP2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   E309D

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. delta-BRIP1-pcDNA3.1/MycHis

   Plasmid

   #19042

   Depositor

   Ronny Drapkin

   Article

   De Nicolo et al Clin Cancer Res. 2008 Jul 15. 14(14):467

   Insert

   delta BRIP1 (BRIP1, BRCA1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   This is a novel BRIP1 germline mutation that cons…

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

9. CC1215

   Bacterial Strain

   #79062

   Purpose

   BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.

   Depositor

   T. J. Kappock

   Bacterial Resistance

   Kanamycin

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pUAP1

    Plasmid

    #63674

    Purpose

    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

    Depositor

    Nicola Patron

    Article

    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

    Insert

    RFP cloning selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBiT3.1-N_PEX7:1-323

    Plasmid

    #211223

    Purpose

    Mammalian expression of full-length human PEX7. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PEX7:M1-A323 (PEX7 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBiT3.1-C_PEX7:1-323

    Plasmid

    #211297

    Purpose

    Mammalian expression of full-length human PEX7. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PEX7:M1-A323 (PEX7 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiT3.1-N_STRAP:1-350

    Plasmid

    #211139

    Purpose

    Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    STRAP:M1-A350 (STRAP Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBiT3.1-C_STRAP:1-350

    Plasmid

    #211180

    Purpose

    Mammalian expression of full-length human STRAP. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    STRAP:M1-A350 (STRAP Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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