We narrowed to 34,718 results for: URE

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pET15-VraS - L114S/D242G

Plasmid

#206873

Purpose

Bacterial Expression of VraS double mutant L114S/D242G

Depositor

May Abdelaziz

Article

Cagle-White et al Arch Biochem Biophys. 2026 Feb;776:11071

Insert

VraS

Tags

6x His

Expression

Bacterial

Mutation

L114S/D242G

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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Lyn-FRB_star-P2A-mVenus-FKBP-5ptase

Plasmid

#241305

Purpose

Mammalian expression of Lyn-FRB_star and mVenus-FKBP-5ptase of INPP5E

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

INPP5E (INPP5E Human)

Tags

mVenus, FKBP

Expression

Mammalian

Mutation

214-644 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E49TAG,G42H,K46H)-mCherry

Plasmid

#241292

Purpose

Mammalian expression of syntaxin-1(E49TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG,G42H,K46H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E53TAG,G42H,K46H)-mCherry

Plasmid

#241294

Purpose

Mammalian expression of syntaxin-1(E53TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG,G42H,K46H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(R210H,E206TAG)-mCherry

Plasmid

#241296

Purpose

Mammalian expression of syntaxin-1(E206TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E206TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(R210H,K84TAG)-mCherry

Plasmid

#241298

Purpose

Mammalian expression of syntaxin-1(K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K84TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(I233A,K84TAG)-mCherry

Plasmid

#241299

Purpose

Mammalian expression of syntaxin-1(I233A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

I233A,K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(I233A,R210H,K84TAG)-mCherry

Plasmid

#241300

Purpose

Mammalian expression of syntaxin-1(I233A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

I233A,K84TAG,R210H

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K264A,K265A,K84TAG)-mCherry

Plasmid

#241301

Purpose

Mammalian expression of syntaxin-1(K264A,K265A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K264A,K265A,K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mVenus-synaptojanin1(498-901)-CAAX

Plasmid

#241303

Purpose

Mammalian expression of truncated form of synaptojanin-1 with CAAX motif fused to mVenus

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Synaptojanin-1 (SYNJ1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

498-901 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS484_10xHis_MBP_TEV_dSpRY

Plasmid

#244832

Purpose

Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS24_10xHis_MBP_TEV_SpRY-Cas9

Plasmid

#244827

Purpose

Bacterial expression of SpRY Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

Plasmid

#244834

Purpose

Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (b)

Plasmid

#204429

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E626A T630A K668A E671A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-2

Plasmid

#204433

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K614A H615A D835A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Palpha6 and Interface-4 (a)

Plasmid

#204434

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

F860A Y861A Y862A Y863A Q864A R865A H866A F867A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-9

Plasmid

#204440

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E213A Q217A R557A D565A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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