We narrowed to 5,839 results for: pCas

1. pCAG-PLE-IRES-mCherry

   Plasmid

   #45766

   Depositor

   Luke Lavis

   Article

   Tian et al Proc Natl Acad Sci U S A. 2012 Mar 27;10

   Insert

   esterase
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Kir2.1-T2A-tdTomato

   Plasmid

   #60598

   Purpose

   Expresses Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Cre-T2A-mRuby2

   Plasmid

   #102989

   Purpose

   Expresses Cre and mRuby2

   Depositor

   Karin Scharffetter-Kochanek

   Article

   Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

   Inserts

   Cre (site-specific recombinase)
   mRuby2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-OSF-PP-human-CHMP3

   Plasmid

   #154180

   Purpose

   expresses human CHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   CHMP3 (CHMP3 Human)
   

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GluA2-Fab-HC-Halo

   Plasmid

   #230061

   Purpose

   Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 Fab heavy chain fragment
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Chicken beta actin (CAG)

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS/ES-EnvA-VSVg-WPRE-pA

   Plasmid

   #87221

   Purpose

   to package wild type EnvA psudotyped lentivirus

   Depositor

   Fan Wang

   Article

   Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

   Insert

   EnvA-VSVg
   

   Use

   Mouse Targeting and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-N-TEV-Twin-Strep

   Plasmid

   #202526

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Tags

   TEV cleavable site and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

   Plasmid

   #128174

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mNaChBac-T2A-tdTomato

    Plasmid

    #60650

    Purpose

    Expresses mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    mNaChBac-T2A-tdTomato
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-IRES2-p75NTR-GFP

    Plasmid

    #182947

    Purpose

    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

    Depositor

    Pantelis Tsoulfas

    Insert

    p75NTR-mGFP
    

    Tags

    HA tag and mGFP

    Expression

    Mammalian

    Mutation

    The leader sequence is from preprotrypin

    Promoter

    CAG

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT378_pattB-pCA-GFP-pA-attB

    Plasmid

    #52554

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS SARS-CoV-2 BA.4/5 Spike

    Plasmid

    #186031

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4/5 Spike (S )
    

    Expression

    Mammalian

    Mutation

    BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVLINKv1-pCALM1-Cre-3' IQGAP3

    Plasmid

    #236705

    Purpose

    3' AAVLINK plasmid for IQGAP3 expression

    Depositor

    Zhonghua Lu

    Insert

    IQGAP3 (IQGAP3 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Promoter

    pCALM1

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-SARS2-S-D614 (C-flag)

    Plasmid

    #156420

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

    Plasmid

    #196197

    Purpose

    Cas9 + gRNA expression to edit G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458-3xHA-SpCas9-HF1

    Plasmid

    #201952

    Purpose

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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