Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,645 results for: pcas9

Clear keywords
Showing: 1581 - 1600 of 2645 results

  2. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135014-plasmid-map-sequence-id-267599

    Enlarge

  3. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135013-plasmid-map-sequence-id-267601

    Enlarge

  10. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid
    #242657
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA, eVRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242657-plasmid-map-sequence-id-488990

    Enlarge

  11. pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971)

    Plasmid
    #242653
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpCas9-VRQR-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, VRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242653-plasmid-map-sequence-id-488991

    Enlarge

  12. CRISPRecise Kit

    Plasmid Kit
    #1000000235
    Purpose
    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.
    Depositor
    Ervin Welker
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  13. gRNA_Cloning Vector BbsI

    Plasmid
    #128433
    Purpose
    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing
    Depositor
    Izuho Hatada
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    July 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    128433-plasmid-map-sequence-id-251645

    Enlarge

  14. Cas9 NGFR

    Plasmid
    #155279
    Purpose
    Lentiviral Cas9 expression with NGFR marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155279-plasmid-map-sequence-id-309983

    Enlarge

  15. Cas9 CFP

    Plasmid
    #155278
    Purpose
    Lentiviral Cas9 expression with CFP marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155278-plasmid-map-sequence-id-309981

    Enlarge

  16. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV/U6
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

    Enlarge

  17. Lenti Cas9/SB100

    Plasmid
    #155294
    Purpose
    Lentiviral expression of Cas9 and SB100
    Depositor
    Martin Carroll
    Insert
    SB100X-P2A-spCas9
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155294-plasmid-map-sequence-id-309998

    Enlarge

  18. Cas9 ROSA sgRNA mCherry

    Plasmid
    #155280
    Purpose
    Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155280-plasmid-map-sequence-id-309987

    Enlarge

Showing: 1581 - 1600 of 2645 results