We narrowed to 5,010 results for: puc

1. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

   Plasmid

   #192350

   Purpose

   L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   AATG_dttLbCas12a-i_TTCG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R; E925Q

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH47811_AvrSr62-7

   Plasmid

   #233534

   Purpose

   Expresses AvrSr62-7 in protoplasts

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-7
   

   Use

   Protoplast expression

   Tags

   Luciferase

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBKT7_AvrSr62-5

   Plasmid

   #233532

   Purpose

   Expresses AvrSr62-5 in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-5
   

   Use

   Tags

   GAL4 BD, Myc tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBKT7_AvrSr62-1

   Plasmid

   #233531

   Purpose

   Expresses AvrSr62-1 in yeast

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   AvrSr62-1
   

   Use

   Tags

   GAL4 BD, Myc tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MPS1 Human

   Plasmid

   #232149

   Purpose

   MPS1 from Human codon optimized for S. cerevisiae in pUCIDT

   Depositor

   Mark Winey

   Article

   Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

   Insert

   MPS1 (TTK Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV10

   Plasmid

   #63213

   Purpose

   pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-PIK3C2A

   Plasmid

   #161988

   Purpose

   Mammalian expression vector containing PIK3C2A tagged with GFP

   Depositor

   Volker Haucke, David Bryant

   Article

   Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

   Plasmid

   #192453

   Purpose

   intronized ttLbCas12a for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
   

   Use

   CRISPR and Synthetic Biology; Moclo compatible le…

   Tags

   Expression

   Mutation

   temperature-tolerant mutation (D156R)

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

   Plasmid

   #158953

   Purpose

   Express human RAD51(T309A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed threonine 309 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNAD1

    Plasmid

    #154347

    Purpose

    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

    Depositor

    John van der Oost

    Article

    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

    Insert

    ShBle
    

    Use

    Microalgae

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Violaxanthin Chlorophyll a Binding Protein Precur…

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

    Plasmid

    #158952

    Purpose

    Express human RAD51(F129A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed phenylalanine 129 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-PH-TAPP1

    Plasmid

    #161985

    Purpose

    Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

    Depositor

    Dario Alessi, David Bryant

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 2xFLAG-2xSTREP EMI1

    Plasmid

    #158947

    Purpose

    Express human EMI1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    EMI1 (FBXO5 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

    Plasmid

    #236421

    Purpose

    transient overexpression of KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    KEAP1 (Keap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

    Plasmid

    #236422

    Purpose

    transient overexpression of KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)

    Plasmid

    #236419

    Purpose

    transient overexpression of EMSY in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    EMSY (2210018M11Rik Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)

    Plasmid

    #236420

    Purpose

    transient overexpression of EMSY in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    EMSY (EMSY Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pITGA5_1745G>U

    Plasmid

    #173812

    Purpose

    mir218 binding site2 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT1745 ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pITGA5_473G>A

    Plasmid

    #173811

    Purpose

    mir218 binding site1 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT473 ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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