We narrowed to 2,618 results for: ubiquitin

1. pGEX6P1-NEDD4L_WW34

   Plasmid

   #187718

   Purpose

   Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-581 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   only residues 492-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-NEDD4L_HECT

   Plasmid

   #187719

   Purpose

   Express GST-tagged NEDD4L HECT domain in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 594-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-593

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmch-mTet1-ZF-CD pc3956

   Plasmid

   #201709

   Purpose

   Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1-ZF_CD (Tet1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Zinc finger domain of Tet1 fused to its catalytic…

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDUET-GST AO7RE ∆238-245

   Plasmid

   #139318

   Purpose

   GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-N-Myc_DW164AA 1-205 USP7

   Plasmid

   #131254

   Purpose

   Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-CMV GFP-LC3B Q116P G120

   Plasmid

   #129290

   Purpose

   Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

   Plasmid

   #119318

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP III (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   C41A, C46A, C840A

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PHAGE-P CMVt N-HA hE6-AP I (G20V)

   Plasmid

   #119308

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (G20V) (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   G20V

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PHAGE-P CMVt N-HA hE6-AP I (C21Y)

   Plasmid

   #119304

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (C21Y) (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   C21Y

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

    Plasmid

    #119313

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 55-376

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)

    Plasmid

    #119317

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP II (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-87

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)

    Plasmid

    #119310

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-64

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PHAGE-P CMVt N-HA hE6-AP I (R39H)

    Plasmid

    #119309

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (R39H) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    G20V

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

    Plasmid

    #119314

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 55-852

    Promoter

    CMV

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 10xHis-ataxin-3 *SIM-HA

    Plasmid

    #89983

    Purpose

    Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    10xHis and HA

    Expression

    Mammalian

    Mutation

    mutated aa 162IFVV to 162AFAA

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-ataxin-3 *SIM

    Plasmid

    #89979

    Purpose

    Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutated 162IFVV to 162AFAA

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PP-UEV1A

    Plasmid

    #25111

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFC0255c:C34858
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. PP-UBC13 (3E95)

    Plasmid

    #25131

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFE1350c:C34878
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. USP15

    Plasmid

    #28282

    Depositor

    Cheryl Arrowsmith

    Insert

    USP15 (USP15 Human)
    

    Use

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    Contains dusp and Ubl domains (residues 6-223).

    Promoter

    Available since

    April 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pLV-SFFV-Emerald-WPRE-UbC-mCherry

    Plasmid

    #211802

    Purpose

    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    Emerald
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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