We narrowed to 7,375 results for: ef1a

1. pREDIT_MCP-RecT

   Plasmid

   #164803

   Purpose

   mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Insert

   RecT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SadCas9 VPR

   Plasmid

   #188514

   Purpose

   Expresses FLAG tagged SadCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LbdCas12a VPR

   Plasmid

   #188513

   Purpose

   Expresses FLAG tagged LbdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSIIhyg-rtTA

   Plasmid

   #139480

   Purpose

   Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   rtTA, Tet-ON reverse transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a promoter

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-LAP2-mCherry

   Plasmid

   #159524

   Purpose

   Expresses mCherry from LAP2 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LAP2

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. DHFR-PP7-VP64_T2A_GFP

   Plasmid

   #86167

   Purpose

   Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-PP7-VP64_T2A_GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized domain (N-terminus version) of E.col…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-eDIO-EYFP

   Plasmid

   #104388

   Purpose

   PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Enhanced Yellow Fluorescent Protein
   

   Use

   Cre/Lox; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas12j2

    Plasmid

    #188498

    Purpose

    Expresses FLAG tagged Cas12j2

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dCas12j2 KRAB

    Plasmid

    #188508

    Purpose

    Expresses FLAG tagged dCas12j2 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXPR_213

    Plasmid

    #133456

    Purpose

    H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    Control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_TMv2_Zeo

    Plasmid

    #131762

    Purpose

    frameshift reporter for barcode retrieval (with Zeo selection)

    Depositor

    Paul Blainey

    Article

    Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-LAP1_2-mCherry

    Plasmid

    #159526

    Purpose

    Expresses mCherry from LAP1_2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LAP1_2

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p23-DDX21 SAT

    Plasmid

    #128804

    Purpose

    overexpression of DDX21 SAT mutation in human cells

    Depositor

    Ling-Ling Chen

    Article

    Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

    Insert

    DDX21 SAT mutation
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p23-DDX21 DEV

    Plasmid

    #128805

    Purpose

    overexpression of DDX21 DEV mutation in human cells

    Depositor

    Ling-Ling Chen

    Article

    Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

    Insert

    DDX21 DEV mutation
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-FDIO-dAPEX2

    Plasmid

    #117175

    Purpose

    Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWPXL-NP1

    Plasmid

    #72654

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    NP1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LbCas12a

    Plasmid

    #188493

    Purpose

    Expresses FLAG tagged LbCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDBEcR/Bla

    Plasmid

    #201556

    Purpose

    Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCF730

    Plasmid

    #121668

    Purpose

    EF1a-ProCas9-Flavi-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    Cas9(C)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCF708

    Plasmid

    #121685

    Purpose

    EF1a-dTEV-protease-mTagBFP2

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    TEV protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

    Plasmid

    #114378

    Purpose

    expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-KA2N-EGFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EGFP and KA2N

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. dCas12j3 KRAB

    Plasmid

    #188507

    Purpose

    Expresses FLAG tagged dCas12j3 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. dCas12j2 VPR

    Plasmid

    #188518

    Purpose

    Expresses FLAG tagged dCas12j2 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

    Plasmid

    #114371

    Purpose

    expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-MBD (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and MBD

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19-EAMP

    Plasmid

    #138265

    Purpose

    mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    iCas9 target-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-HTLV

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

    Plasmid

    #114374

    Purpose

    expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag‐Tim2

    Plasmid

    #49213

    Purpose

    expresses Flag tagged Tim2

    Depositor

    Lawrence Kane

    Article

    Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

    Insert

    Tim2 (Timd2 Mouse)
    

    Use

    Tags

    CD8 signal sequence and FLAG

    Expression

    Mammalian

    Mutation

    no Tim2 signal sequence; R80L and S196D compared …

    Promoter

    EF1a

    Available Since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

30. rAAV-Ef1α-TdTomato-WPRE-polyA

    Plasmid

    #241828

    Purpose

    AAV construct for constitutive expression of TdTomato.

    Depositor

    Benjamin Arenkiel

    Article

    Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

    Insert

    TdTomato-WPRE-polyA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge