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We narrowed to 3,270 results for: Streptococcus

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Showing: 1601 - 1620 of 3270 results

  2. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

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  3. pCjedCas9

    Plasmid
    #172216
    Purpose
    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells
    Depositor
    John van der Oost
    Publication
    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    Tags
    Histag
    Mutation
    changed Asp 8 to Ala and His 559 to Ala
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    172216-plasmid-map-sequence-id-338133

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  4. SpRY_ATH51-IP

    Plasmid
    #235991
    Purpose
    Expresses SpRY_ATH51 in mammalian cells
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpRY_ATH51
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    ectopic insertion of 7 amino acids of PID turn-he…
    Promoter
    EF-1a
    Available since
    Aug. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    235991-plasmid-map-sequence-id-469268

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  5. pTox-EGFPsite1_NGA-PAM (BPK754)

    Plasmid
    #181747
    Purpose
    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
    Use
    Selection plasmid
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181747-plasmid-map-sequence-id-358237

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  6. pLCHKO_intergenic-intergenic_1

    Plasmid
    #155077
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155077-plasmid-map-sequence-id-308535

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  8. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  12. pLCHKO_Ptbp_ex8_1

    Plasmid
    #155081
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155081-plasmid-map-sequence-id-308533

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  13. pLCHKO_Ptbp_ex8_4

    Plasmid
    #155084
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155084-plasmid-map-sequence-id-308527

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  14. pLCHKO_Ptbp_ex8_2

    Plasmid
    #155082
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155082-plasmid-map-sequence-id-308510

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  15. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Publication
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

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  16. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  18. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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Showing: 1601 - 1620 of 3270 results