Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,540 results for: T2A

Clear keywords
Showing: 1601 - 1620 of 2540 results

  1. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

    Enlarge

  15. pYPQ127B

    Plasmid
    #99883
    Purpose
    Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    TALE-VP64-T2A-TALE-VP64
    Use
    TALEN
    Expression
    Plant
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    99883-plasmid-map-sequence-id-203133

    Enlarge

  16. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

    Enlarge

  19. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

    Enlarge

  20. pSSA-RPG

    Plasmid
    #85932
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85932-plasmid-map-sequence-id-172472

    Enlarge

Showing: 1601 - 1620 of 2540 results