We narrowed to 4,339 results for: TIL

1. pcDNA3.1-Rho*-BERKY3

   Plasmid

   #158220

   Purpose

   BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV TetR Blast (716-1)

   Plasmid

   #17492

   Purpose

   3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Tetracycline repressor
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriExEKAR2G2

   Plasmid

   #40175

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-IRES-Gβγ-BERKY3

   Plasmid

   #158426

   Purpose

   BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-3HA-His3MX6

   Plasmid

   #41600

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-IRES-Gαq*-BERKY3

   Plasmid

   #158425

   Purpose

   BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBMNb-cALFA

   Plasmid

   #159991

   Purpose

   For the protein expression with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   CMV promoter, WPRE

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVUT-tTR-KRAB

    Plasmid

    #11651

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Has Service

    Cloning Grade DNA

    Insert

    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid

    #45574

    Purpose

    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    co I-Sce I
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP FAK

    Plasmid

    #50515

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

    Insert

    protein tyrosine kinase
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLRP3-mNeonGreen

    Plasmid

    #200942

    Purpose

    NLRP3 fused with mNeonGreen to indicate its localization change

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-LCN2

    Plasmid

    #28225

    Depositor

    Sebastien Jauliac

    Article

    Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

    Insert

    LCN2 promoter (LCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    LCN2

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

16. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti PGK GFP Puro (w509-5)

    Plasmid

    #19070

    Purpose

    3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-OTp-GCaMP6s

    Plasmid

    #192945

    Purpose

    To drive GCaMP6s under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. NAX_CAGGS_CAS9_Blasto

    Plasmid

    #167856

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge