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CSIV-DEST

Plasmid

#141053

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R4

Plasmid

#141054

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pHygTK

Plasmid

#141059

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HygTK

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R5R4

Plasmid

#141055

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pPuroTK

Plasmid

#141060

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R4-H1-BsmBI-L2

Plasmid

#141034

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-EF1_-R3

Plasmid

#141037

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EF1_ promoter

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R4-H1-BbsI-L2

Plasmid

#141033

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-U6-BbsI-R5

Plasmid

#141031

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9n-L2

Plasmid

#141039

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9n

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-CMV-R3

Plasmid

#141035

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

CMV promoter

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R2L4

Plasmid

#141061

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_med

Plasmid

#128849

Purpose

Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_hi

Plasmid

#128853

Purpose

High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YSBE415: pTOP_ACA1_Vc_HOG1 terminator

Plasmid

#122434

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

C-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE416: pTOP_CAC1_Vn_HOG1 terminator

Plasmid

#122435

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

N-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-amo

Plasmid

#113190

Purpose

Expresses amo in vertebrate cells

Depositor

Craig Montell

Article

Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

Insert

amo (Pkd2 Fly)

Use

In vitro transcription

Expression

Mammalian

Mutation

Y602H

Promoter

simian CMV IE94

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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p236_LTJ_sgRNAFoxp3sf/J

Plasmid

#82676

Purpose

sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 sf/J

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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