We narrowed to 2,530 results for: Kap

1. pLKO.1 mTagBFP2 rat DJ-1 shRNA

   Plasmid

   #222870

   Purpose

   Expresses mTagBFP2 along with an shRNA against rat DJ-1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   DJ-1 shRNA (Park7 Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Duet-6xHis-TEV-Nsp8(76-198)

   Plasmid

   #201024

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTagGFP2-N SIN1 Na

   Plasmid

   #124928

   Purpose

   For expression of a.a. 1-68 of SIN1-GFP

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MAPKAP1 (MAPKAP1 Human)
   

   Use

   Tags

   TagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FNE2-A72

   Plasmid

   #44608

   Depositor

   Robert Oshima

   Article

   Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

   Insert

   Ets2-A72 (Ets2 Mouse)
   

   Use

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   THR-72 converted to Alanine

   Promoter

   CMV

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. FUW-TetO-Klf5

   Plasmid

   #128830

   Purpose

   dox-dependent expression of mouse Klf5

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Klf5 (Klf5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHTN_HaloTag_KIF5B

   Plasmid

   #213404

   Purpose

   Full-length human kinesin-1 with HaloTag at the N-terminus

   Depositor

   Jonas Ries

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   HaloTag-KIF5B (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-DeAct-GS1

   Plasmid

   #89445

   Purpose

   Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

   Plasmid

   #159107

   Purpose

   The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

   Depositor

   Thomas Tuschl

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   NSP12
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetO-Gata6

    Plasmid

    #128827

    Purpose

    dox-dependent expression of mouse Gata6

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMC00035 (aka pMG0784)

    Plasmid

    #210768

    Purpose

    Lentiviral vector to express human ADRM1

    Depositor

    Raj Chari

    Article

    Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

    Insert

    ADRM1 (ADRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHTN_HaloTag_KIF5B[1-560]

    Plasmid

    #213403

    Purpose

    Truncated human kinesin-1 with HaloTag at the N-terminus

    Depositor

    Jonas Ries

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    HaloTag-KIF5B[1-560] (KIF5B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-Trim46

    Plasmid

    #176402

    Purpose

    TRIM46 tagged with GFP

    Depositor

    Casper Hoogenraad, Thomas Schwarz

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-Trim46

    Plasmid

    #176401

    Purpose

    TRIM46 tagged with mCherry

    Depositor

    Casper Hoogenraad, Thomas Schwarz

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTetON-DeAct-GS1

    Plasmid

    #89454

    Purpose

    Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-GS1 (GSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning cytoplasmic Gsn amino acids 5…

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTagGFP2-N SIN1 deltaNCD

    Plasmid

    #124920

    Purpose

    For expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137)

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330-Elf5 gRNA

    Plasmid

    #128836

    Purpose

    gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    mElf5 gRNA (Elf5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-Blast POLD1

    Plasmid

    #160805

    Purpose

    Express POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon optimized C-terminal 230 amino acids

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

    Plasmid

    #62977

    Purpose

    Expresses myc-tagged mouse βTrCP residues 241-569

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codes for residues 241-569

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

    Plasmid

    #128835

    Purpose

    Knock-in of TagBFP into mouse Esrrb locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    tagBFP into Esrrb locus (Esrrb Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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