We narrowed to 24,008 results for: NUC

1. pLV-EF1a-Tmx4-V5

   Plasmid

   #120236

   Purpose

   Expresses V5-tagged Tmx4 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Tmx4 (Tmx4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. VirTREX2-HLDel-1_NbATML1-1pro

   Plasmid

   #231149

   Purpose

   T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1pro

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   TREX2 and mobile gRNA targeting NbATML1-1pro
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. VirTREX2-HLDel-1_NbSTM-5'UTR

   Plasmid

   #231148

   Purpose

   T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTR

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   TREX2 and mobile gRNA targeting NbSTM-5?UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. esgRNA_NbATML1-2pro and NbATML1-1pro

   Plasmid

   #231155

   Purpose

   T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC2.4

   Plasmid

   #226557

   Purpose

   NATURA gene encoding plasmid

   Depositor

   Dave Wee

   Article

   Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

   Insert

   NATURA reporter gene
   

   Use

   Luciferase; Piggybac

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACE SOX (ORF37) D221N E244Q

   Plasmid

   #192053

   Purpose

   Expresses a 2X catalytic mutant of (ORF37) D221N E244Q

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (D221N E244Q)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   2X catalytic mutant D221N E244Q

   Promoter

   CMV

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-DmPop2a-E151A-dsRNAres-V5His6_R

   Plasmid

   #147363

   Purpose

   Insect Expression of DmPop2a-E151A-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

   Insert

   DmPop2a-E151A-dsRNAres (Pop2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmPop2a-E162A-dsRNAres-V5His6_R

    Plasmid

    #147364

    Purpose

    Insect Expression of DmPop2a-E162A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-E162A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmPop2a-S155A-dsRNAres-V5His6_R

    Plasmid

    #147365

    Purpose

    Insect Expression of DmPop2a-S155A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-S155A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnEA-NpG-HsNot7_R

    Plasmid

    #147366

    Purpose

    Bacterial Expression of HsNot7

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot7 (CNOT7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmPop2a-D53AE55AE151A-dsRNAres-V5His6_R

    Plasmid

    #147362

    Purpose

    Insect Expression of DmPop2a-D53AE55AE151A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-D53AE55AE151A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRD393

    Plasmid

    #168774

    Purpose

    Expression of mEos3.2_H-NSdbd in Mycobacterium

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    mEos3.2_H-NSdbd
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-Hypa-nCas9n-A69

    Plasmid

    #125645

    Purpose

    micro-injection of poly-adenylated Hypa-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    Hypa-nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    N692A, M694A, Q695A, H698A from wild-type Cas9

    Promoter

    T3

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-HA-MRE11-H129N

    Plasmid

    #82034

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Nuclease-dead mutant: H129N MRE11

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICE-HA-MRE11-H63D

    Plasmid

    #82036

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exonuclease-dead mutant: H63D MRE11

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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