We narrowed to 1,687 results for: plasmid for e coli

1. E. coli MEV15

   Bacterial Strain

   #197112

   Purpose

   E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

2. pHis-TEV-Nsp15-E266Q

   Plasmid

   #218803

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   E266Q (G to C)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHis-TEV-Nsp15-V66L

   Plasmid

   #219524

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   V66L (G to C)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHis-TEV-Nsp15-A81V

   Plasmid

   #219525

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   A81V (C to T)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHis-TEV-Nsp15-D183N

   Plasmid

   #219526

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   D183N (G to A)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHis-TEV-Nsp15-H234A

   Plasmid

   #219529

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   H234A (CAC to GCG)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGST-HsSyx-375-792

   Plasmid

   #179292

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + GST + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 375-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSUMO-HsSyx3

   Plasmid

   #179288

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + SUMO + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-hMICU1EF

   Plasmid

   #199180

   Purpose

   This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

   Depositor

   Ming-Feng Tsai

   Article

   Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   MICU1 (MICU1 Human)
   

   Use

   Tags

   His6 and MBP, Thrombin site, TEV site

   Expression

   Mutation

   D421A, E432K, F433W, F453W

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP-HsSyx3

    Plasmid

    #179289

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + GFP + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHsSyx3-MBP

    Plasmid

    #179286

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    TEV protease cleavage site + mature maltose bindi…

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET3a-SF-LMNC-TS

    Plasmid

    #169374

    Purpose

    E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

    Depositor

    Bernd Bukau

    Article

    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

    Insert

    LMNA residues 31-542 (LMNA Human)
    

    Use

    Tags

    Split-FlAsH Tag and TwinStrep Tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-30 (unstructured head domain…

    Promoter

    Native pET3a promotor

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIC-ZB-SPRTN-E112A-FL

    Plasmid

    #110218

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNIC-ZB-SPRTN-Y117C-FL

    Plasmid

    #110219

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    Y117C, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 7AA-aSyn-pRK172

    Plasmid

    #236197

    Purpose

    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.

    Depositor

    Sjors Scheres, Yang Yang

    Article

    Yang et al Acta Neuropathol. 2023 May;145(5):561-57

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insertion os MAAAEKT at residue 22

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBP-HsSyx3

    Plasmid

    #179287

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMBP-HsSyx-375-792

    Plasmid

    #179291

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET3d-SF-DCTN1-TS

    Plasmid

    #169375

    Purpose

    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

    Depositor

    Bernd Bukau

    Article

    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

    Insert

    dynactin subunit 1 isoform 1 (DCTN1 Human)
    

    Use

    Tags

    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

    Expression

    Bacterial

    Mutation

    Point mutations: A139T, I304T, Q322R, T516A, E577…

    Promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS1153

    Plasmid

    #29068

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple36 (CRH Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge