We narrowed to 1,719 results for: plasmid for e coli

1. pRSETa-mKikGR-V69T

   Plasmid

   #98574

   Purpose

   photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mKikGR-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLei-sfGFP-V2*D134*Y152*

   Plasmid

   #191112

   Purpose

   An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing V2 & D134 & Y152 to TAG

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-PRD(703-868, P801G)

   Plasmid

   #180029

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   P801G

   Promoter

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-MBP-DQNAT

   Plasmid

   #198215

   Purpose

   pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   MBP_DQNAT
   

   Use

   Tags

   6x His Tag and DQNAT glycosylation tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)

   Plasmid

   #191646

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

   Plasmid

   #160540

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Inserts

   SARS-CoV-2 NSP7 (ORF1ab Synthetic)
   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   SARS-CoV-2 NSP12 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pExp-RBD-CHis

   Plasmid

   #195002

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

   Plasmid

   #192822

   Purpose

   Golden Gate compatible plasmid with mCherry and Neo

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   mCherry and Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMWCAVI_H6BAP-OROV_N

   Plasmid

   #240162

   Purpose

   Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli

   Depositor

   Stephen Graham

   Article

   Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

   Insert

   Nucleoprotein
   

   Use

   Tags

   6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEVOL-NBK-1

    Plasmid

    #207639

    Purpose

    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The evolved synthetase NBK-1 contains mutations Y…

    Promoter

    araBAD

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

    Plasmid

    #191650

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MERS-coV-NSP3-c001

    Plasmid

    #173085

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    D1109-D1275, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. B8-pVTCB052

    Plasmid

    #231302

    Purpose

    Plasmid used for expression of AI-designed GFP protein B8 in E. coli

    Depositor

    Alexander Rives

    Insert

    B8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

    Plasmid

    #191651

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-optimized E6AP F727D

    Plasmid

    #165101

    Purpose

    ecoli optimized expression of a GST fusion of Human E6AP F727D

    Depositor

    Arthur Haas

    Article

    Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

    Insert

    E6AP F727D (UBE3A Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234454

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234441

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Has Service

    AAV1

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234442

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Has Service

    AAV1

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234453

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid

    #225617

    Purpose

    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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