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pCAG-3xFLAG-BioID2-MCS

Plasmid

#186811

Purpose

CAG promoter-driven expression of BioID2-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

3xFLAG-BioID2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-pT7native

Plasmid

#200002

Purpose

Violacein pathway. pT7-native CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
pDIV296

Plasmid

#204687

Purpose

AMA1 plasmid with nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

nat1

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-native

Plasmid

#200001

Purpose

Violacein pathway. Native promoter and CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP1 N-terminal truncation

Plasmid

#22404

Purpose

Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1 (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pDIV297

Plasmid

#204820

Purpose

AMA1 plasmid with amdS selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

amdS

Use

Fungal expression

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans trpC promoter

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-RGECO1.0

Plasmid

#175813

Purpose

The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

RGECO1.0

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a - NR

Plasmid

#89625

Purpose

Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Nitrate reductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)

Plasmid

#72273

Purpose

FLAG-Mnase expressed under the REB1 promoter

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Promoter

REB1

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pQC-SEAP #M004#725

Plasmid

#42125

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

alkaline phosphatase, placental (SEAP) (ALPP Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pKS1

Plasmid

#24636

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pTYF-EF1a-DLDH-IRES-EGFP

Plasmid

#178310

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - GR

Plasmid

#89614

Purpose

Expresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Glutaredoxin

Tags

6HIS

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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