We narrowed to 12,972 results for: lic

1. pETM30-C9ORF78_R41A

   Plasmid

   #193368

   Purpose

   Expression of C9ORF78 R41A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

   Depositor

   Markus Wahl

   Article

   Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

   Insert

   C9ORF78_R41A (C9orf78 Human)
   

   Use

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   R41A, the mutation is located in the binding inte…

   Promoter

   T7

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_B2M_sgRNA(PP7)

   Plasmid

   #232438

   Purpose

   sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-ND-Cdt1Δ499-546-HA-Puro

   Plasmid

   #193768

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1Δ499-546 (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF-MCM3/5

   Plasmid

   #116950

   Purpose

   Expresses human MCM3 and human MCM5

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM5 (MCM5 Human)
   human MCM3 (MCM3 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-MCM4/6

   Plasmid

   #116951

   Purpose

   Expresses human MCM4 and human MCM6

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM6 (MCM6 Human)
   Human MCM4 (MCM4 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-HA-RAD52-∆C

   Plasmid

   #235677

   Purpose

   Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 302-410

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET32-MCM2/7

   Plasmid

   #116949

   Purpose

   Expresses human MCM2 and human MCM7

   Depositor

   James Chong

   Article

   Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

   Inserts

   Human MCM7 (MCM7 Human)
   Human MCM2 (MCM2 Human)
   

   Use

   Tags

   Thioredoxin, His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SEPT_Cdk2-T160A-siR

   Plasmid

   #73975

   Purpose

   HDR template to generate Cdk2-T160A-siR

   Depositor

   Manuel Kaulich, Steven Dowdy

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160A (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Ala and siRNA target site mutated

   Promoter

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-mCherry-2A-BRD4 Iso AdelCTD

   Plasmid

   #137722

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated at amino acid 1328

   Promoter

   Tight TRE promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid

    #137723

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 600-682

    Promoter

    Tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC-NMYC-hTRF1deltaBLM

    Plasmid

    #64165

    Purpose

    Retroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deleted amino acids aa317-374 of human TRF1

    Promoter

    CMV

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-SEPT_Cdk2-T160E-siR

    Plasmid

    #73976

    Purpose

    HDR template to generate Cdk2-T160E-siR

    Depositor

    Manuel Kaulich, Steven Dowdy

    Article

    Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

    Inserts

    Cdk2-LHA-siR (CDK2 Human)
    Cdk2-RHA-T160E (CDK2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    T160 mutated to Glu and siRNA target site mutated

    Promoter

    Available since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX209-neo-WRN K577M

    Plasmid

    #125790

    Purpose

    constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-WRN K577M (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    K577M (helicase-dead)

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX209-neo-WRN E84A K577M

    Plasmid

    #125791

    Purpose

    constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-WRN E84A/K577M (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M (exonuclease/helicase-dead)

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL Neo Myr Flag CAMK1G

    Plasmid

    #20438

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CAMK1G (CAMK1G Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 6A NLRP1 CARD-FLAG WT

    Plasmid

    #163978

    Purpose

    Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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