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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 19,599 results for: NSI

Showing: 3321 - 3360 of 19599 results

  1. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

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  2. U2m

    Plasmid
    #121015
    Purpose
    MoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD11
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121015-plasmid-map-sequence-id-236174

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  3. U3m

    Plasmid
    #121016
    Purpose
    MoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD13
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121016-plasmid-map-sequence-id-236176

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  4. U5m

    Plasmid
    #121018
    Purpose
    MoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD14
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121018-plasmid-map-sequence-id-238061

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  5. U6m

    Plasmid
    #121019
    Purpose
    MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD20
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121019-plasmid-map-sequence-id-238082

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  6. U7m

    Plasmid
    #121020
    Purpose
    MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD16
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121020-plasmid-map-sequence-id-236180

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  7. U8m

    Plasmid
    #121021
    Purpose
    MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD24
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121021-plasmid-map-sequence-id-236184

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  8. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

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  9. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  10. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  11. C59m

    Plasmid
    #120976
    Purpose
    MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mScarlet
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120976-plasmid-map-sequence-id-236030

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  12. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

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  13. C1m

    Plasmid
    #120955
    Purpose
    MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)
    Depositor
    Richard Murray
    Insert
    deGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120955-plasmid-map-sequence-id-235968

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  14. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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  15. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

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  16. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

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  17. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  18. V37m

    Plasmid
    #121043
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and mScarlet selection
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121043-plasmid-map-sequence-id-236355

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  19. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  20. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  21. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  22. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  23. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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  24. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  25. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  26. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

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  27. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  28. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  29. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

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  30. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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  31. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

    Enlarge

  32. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  33. pAAV-hSyn-GRAB_NTS1.0

    Plasmid
    #180006
    Purpose
    Expresses the neurotensin sensor GRAB_NTS1.0 in neurons
    Depositor
    Yulong Li
    Insert
    neurotensin sensor GRAB_NTS1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    180006-plasmid-map-sequence-id-358494

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  34. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

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  35. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

    Enlarge

  36. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  37. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

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  38. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

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  39. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

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  40. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

    Enlarge

Showing: 3321 - 3360 of 19599 results