We narrowed to 3,658 results for: pb
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Plasmid#192823PurposeExpresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4DepositorUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
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pb-miRE-tre-3xFLAG-hnRNPU∆RGG
Plasmid#196088PurposeExpresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cellsDepositorInsertSAF-A (Hnrnpu Mouse)
UseTagsN-terminal 3xFLAGExpressionBacterial and MammalianMutationremoved last 154 amino acids on cterminal endPromoterTREAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-GS
Plasmid#188939PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-GS
Plasmid#188938PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-DHFR-3xHA
Plasmid#188934PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-DHFR-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G
Plasmid#146359PurposeInsect Expression of DmDCP2-PBDepositorInsertDmDCP2-PB (DCP2 Fly)
UseTagsExpressionInsectMutationContains an in-frame insertion that adds a Q afte…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-KRAB-dCas9
Plasmid#120357PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-sfGFP-GSAx9-iCre-ERT2
Plasmid#137857PurposeCAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposonDepositorInsertsfGFP-GSAx9-iCre-ERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS CMV NA 3C FLAG SA
Plasmid#234993PurposeExpression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERP2-(N-HA)KRAS_G12D
Plasmid#201254PurposeDox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cellsDepositorInsertKRAS (KRAS Human)
UsePiggybac transposonTagsHA-TagExpressionMammalianMutationchanged Gly (G) 12 to Asp (D)PromoterTREAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-mRuby3-Gal8-P2A-Zeo
Plasmid#150815PurposeMammalian expression of mRuby3-Galectin8 (Gal8) fusion proteinDepositorInsertmRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)
UseTagsGal8 is fused to the c-terminus of mRuby3ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
iF53 PB-Zim3-dCas9-mScarlet-Hygro
Plasmid#204726PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1afull-H2B-mCherry-P2A-Fluc
Plasmid#207253PurposeLentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoterDepositorInsertH2B-mCherry-P2A-Fluc
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alpha_fullAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mNeonGreen
Plasmid#175508PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-PG13-mCherry-EF1a-PuroR-EGFP
Plasmid#195292PurposepiggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)DepositorInsertsmCherry
PuroR-P2A-EGFP
UsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
EK0379 CMV-TO-EGFP-stop-t70587 (PB)
Plasmid#191154PurposeExpression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.DepositorInsertEGFP-t70587
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only