We narrowed to 4,338 results for: BFP

1. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-AID-BLM-UGI-T2A-mTagBFP2

   Plasmid

   #221222

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-BLM-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

   Plasmid

   #221223

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-Ns3h-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

   Plasmid

   #173029

   Purpose

   For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   pspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, BFP, HIV NES, and T2A

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

   Plasmid

   #171588

   Purpose

   Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   Bxb1 integrase
   iCasp9
   BFP
   rtTA3G
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS DRR mCherry Donor EF1a BFP

    Plasmid

    #98896

    Purpose

    Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-mCherry-GFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

    Plasmid

    #205161

    Purpose

    express β-ACTIN-3′UTR-24xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

    Plasmid

    #242407

    Purpose

    Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

    Depositor

    Ewan St. John Smith

    Article

    Lim et al bioRxiv 2024.09.24.614636

    Inserts

    mScarlet3
    IRES-EGFP
    IRES-moxBFP
    

    Use

    Self-amplifying rna

    Expression

    Mammalian

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-2-TagBFP-SL2-Pre-SPHERE-SF-iGluSnFR

    Plasmid

    #187099

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-SF-iGluSnFR
    

    Tags

    TagBFP-SL2

    Expression

    Worm

    Promoter

    Str-2

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV anti-FITC-LaG16-Y186A-T2A-BFP

    Plasmid

    #194932

    Purpose

    LV construct encoding Y186A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Promoter

    TRE, PGK

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211462

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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