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pHS1871
Plasmid#205976PurposeU6-JAGBLH010000220 Cas8-HNH DR-BpiI-DRDepositorInsertCas8-HNH DR-BpiI-DR
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP
Plasmid#198352PurposePart of Firefly reporter system, encodes Renilla luciferaseDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Integrin-Beta1-N-18
Plasmid#55303PurposeLocalization: Integrin/Focal Adhesions, Excitation: 399, Emission: 456DepositorInsertIntegrin-Beta1 (ITGB1 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
UseTags14xHis on Csf3ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1517
Plasmid#205980PurposeFLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNATM3.1/V5-His TOPO-PKCi-K274W
Plasmid#55690Purposeexpresses dominant negative human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120E and K274WPromoterCMVAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(8G)-mCherry
Plasmid#206950PurposeMammalian expression of heterobifunctional SasG linkers (8G)DepositorInsertsfGFP-SaSasG (8G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pHS1516
Plasmid#205979PurposeFLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas7 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1477
Plasmid#205970PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)DepositorInsertCas5-HNH all proteins, with His-tagged Cas11
UseTags14xHis on Cas11ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Zyxin-6
Plasmid#57802PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertZyxin (ZYX Human)
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-A120E
Plasmid#55687Purposeexpresses constitutively active human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120EPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTU387
Plasmid#178981PurposepACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR arrayDepositorInsertCRISPR array from Candidatus Scalindua brodae
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
