We narrowed to 7,486 results for: CIT

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pET21d-colEdes6/Imdes6

Plasmid

#107114

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes6/Imdes6

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Gm1

Plasmid

#53528

Purpose

Expresses a plant arginyl-tRNA synthetase in E.coli

Depositor

Gabor Igloi

Article

Igloi et al FEBS J. 2009 Mar;276(5):1307-18. doi: 10

Insert

arginyl-tRNA synthetase

Tags

His Tag and Thioredoxin

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCY 3120-01

Plasmid

#36237

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yECitrine-7Myc

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-7Myc-kanMX

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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phtsResA

Plasmid

#25914

Depositor

Johann Deisenhofer

Article

Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

Insert

His-tagged soluble ResA (resA Bacillus subtilis)

Tags

His

Expression

Bacterial

Mutation

Start at Serine 37 to remove n-terminal transmemb…

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
AIRAP 2: AIRAP(m1.9-T7)pGEX.KG

Plasmid

#21792

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Sup35 Sc/Ca NM Chimera S17R His7 Cys

Plasmid

#15601

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Candida albicans, Budding Yeast)

Tags

7xHis and Cysteine

Expression

Bacterial

Mutation

S17R. Serine 17 mutated to arginine.

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

Plasmid

#14849

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

Plasmid

#14850

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Tags

ECFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
AB65_pCAG.Cas9-D10A.rBGpA

Plasmid

#199256

Purpose

Expression construct encoding the SpCas9-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SpCas9.D10A nickase

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Article

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_11

Plasmid

#60582

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_11

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Cre

Plasmid

#123133

Purpose

Mammalian expression vector for wild-type Cre.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Cre recombinase

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1553 - pAAV EF1a EGFP-KASH

Plasmid

#131682

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Insert

EGFP-KASH

Use

AAV

Tags

KASH

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CW3SL-EGFP

Plasmid

#61463

Purpose

AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

Depositor

Bong-Kiun Kaang

Article

Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

Insert

EGFP

Use

AAV

Promoter

CaMKIIa

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-sRGN3.1-BlastR

Plasmid

#220964

Purpose

Expresses human codon-optimized sRGN3.1

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

sRGN3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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