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pEAHISPSVORF137

Plasmid

#97023

Purpose

Expressing Pyrobaculum spherical virus orf137 protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 137

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF131

Plasmid

#97022

Purpose

Expressing Pyrobaculum spherical virus orf131 protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 131

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet-6xHis-Strep-CtVPS13 (1-635)

Plasmid

#248331

Purpose

Bacterial expression of a N-temrinal fragment of VPS13 from C. Thermophilum for in vitro studies

Depositor

Karin Reinisch

Publication

Li et al Mol Cell. 2026 Jul 7:S1097-2765(26)00417

Insert

VPS13

Tags

6xHis

Expression

Bacterial

Mutation

Contains residues 1-635 only

Promoter

T7

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_35-250-del183-202_AF

Plasmid

#148721

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Publication

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_40-250-del183-202_AF

Plasmid

#148723

Purpose

Bacterial Expression of CteIF4E_40-250-del183-202

Depositor

Elisa Izaurralde

Publication

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_40-250-del183-202

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CteIF4E_35-250-del183-202_AF

Plasmid

#148725

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Publication

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mNeonAce-NeuroD

Plasmid

#253617

Purpose

Zebrafish neuronal subset expression of Ace2N-mNeon for voltage imaging

Depositor

Daan Brinks

Publication

Wu et al Neurophotonics. 2026 Feb;13(Suppl 2):S23

Insert

Ace2N-mNeon

Use

Zebrafish expression

Promoter

neuroD

Available since

Aug. 26, 2026

Availability

Academic Institutions and Nonprofits only

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mNeonAce-acta2

Plasmid

#253618

Purpose

Zebrafish smooth muscle/pericytes expression of Ace2N-mNeon for voltage imaging

Depositor

Daan Brinks

Publication

Wu et al Neurophotonics. 2026 Feb;13(Suppl 2):S23

Insert

Ace2N-mNeon

Use

Zebrafish expression

Promoter

acta2

Available since

Aug. 10, 2026

Availability

Academic Institutions and Nonprofits only

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mNeonAce-unc45

Plasmid

#253619

Purpose

Zebrafish skeletal muscle/adaxial expression of Ace2N-mNeon for voltage imaging

Depositor

Daan Brinks

Publication

Wu et al Neurophotonics. 2026 Feb;13(Suppl 2):S23

Insert

Ace2N-mNeon

Use

Zebrafish expression

Promoter

unc45

Available since

Aug. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2pA2_cmlc2_Ace2N-mNeon

Plasmid

#253664

Purpose

Zebrafish cardiomyocytes expression of Ace2N-mNeon for voltage imaging

Depositor

Daan Brinks

Publication

Wu et al Neurophotonics. 2026 Feb;13(Suppl 2):S23

Insert

Ace2N-mNeon

Use

Zebrafish expression

Promoter

cmlc2

Available since

Aug. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC_Rho-tag-Pro30-mEGFP-NLS3x

Plasmid

#249671

Purpose

Expression of rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish

Depositor

Kai Johnsson, Richard Wombacher

Publication

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag-Pro30-mEGFP-NLS3x

Use

Zebrafish expression

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC_SiR-tag-Pro30-mEGFP-NLS3x

Plasmid

#249672

Purpose

Expression of silicon rhodamine-binding protein tag fused to mEGFP (nuclear) in zebrafish

Depositor

Kai Johnsson, Richard Wombacher

Publication

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag-Pro30-mEGFP-NLS3x

Use

Zebrafish expression

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLV_TRE3G_hRTN3E-Halo

Plasmid

#216252

Purpose

Tet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vector

Depositor

Edward Avezov

Publication

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon-3 isoform e (RTN3 Human)

Use

Lentiviral

Tags

HaloTag

Promoter

TRE3G

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLL1271

Plasmid

#129282

Purpose

Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)

Depositor

Lee Lynd

Insert

alpha-Xylp_1211 (Ga0256695_ 1211)

Expression

Bacterial

Promoter

Clostridium thermocellum DSM1313 Cellobiose Phosp…

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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AtALMT1-uPIC 5'

Plasmid

#117886

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

AtALMT1-uPIC 5' (ALMT1 Mustard Weed)

Expression

Yeast

Promoter

AOX

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AtALMT1-uPIC 3'

Plasmid

#117888

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

AtALMT1-uPIC 3' (ALMT1 Mustard Weed)

Expression

Yeast

Promoter

AOX

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-Spec(x2)-DocI

Plasmid

#58713

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Publication

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Spec(x2)-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pUPF1-N-term-AID-V5-P2A-BSD

Plasmid

#251489

Purpose

AID N-terminal UPF1 targeting vector

Depositor

Markus Landthaler

Publication

Boehm et al Mol Cell. 2025 Sep 18;85(18):3524-3546.e

Insert

Homology arms for N-terminal targeting of UPF1 with AID-BSD cassette

Tags

auxin-induced degron tag

Expression

Bacterial

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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GB4542

Plasmid

#215261

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

Depositor

Diego Orzaez

Publication

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Bral1 scFv [N364/42 scFv]

Plasmid

#219494

Purpose

Mammalian Expression Plasmid of anti-Bral1 (Mouse) scFv. Derived from hybridoma N364/42.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Bral1 (Mus musculus) recombinant mouse scFv. (Hapln2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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