We narrowed to 5,844 results for: pcas

1. pCAGGS-pac-T2A-FLPo

   Plasmid

   #124835

   Purpose

   Puromycin resistance gene and FLP recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-FLPo
   

   Expression

   Mammalian

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Tet-on Advanced -BGH-PA

   Plasmid

   #114379

   Purpose

   Express reverse tetracycline inducible rtTA transcriptional activator

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

   Insert

   rtTA and BGH-pA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS D614G soluble Spike

   Plasmid

   #164651

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-AMPH1-BAR-mCherry

   Plasmid

   #85130

   Purpose

   BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cmv d0 dPspCas13b-GGS-NYTHDF1

   Plasmid

   #119855

   Purpose

   Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

   Depositor

   Bryan Dickinson

   Article

   Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

   Insert

   dCas13b-GGS-NYTHDF1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pCaMKII-EBFP-tTAn

   Plasmid

   #172124

   Purpose

   Expression of EBFP and tTA-InteinN in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, EBFP, tTAn-InteinN
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-tagRFP-NLS-HA-Bxb1

   Plasmid

   #65625

   Purpose

   Vector for cotranslational expression of tagRFP-Bxb1

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Bxb1 integrase
   

   Tags

   HA, SV40 NLS, and tagRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-CRY2-mCherry-MYPT169

   Plasmid

   #178526

   Purpose

   Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry-MYPT169
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG mFoxg1-GFP wildtype

   Plasmid

   #73048

   Purpose

   C-terminal fusion of gfp Mouse wildtype Foxg1

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   mFoxg1 (Foxg1 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid

    #113837

    Purpose

    Monomeric TagBFP2 and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCre
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS D614 soluble Spike

    Plasmid

    #164652

    Purpose

    Expresses SARS-CoV-2 soluble Spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 soluble spike (S SARS-CoV-2)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-Flag-hsDicer (D1709A)

    Plasmid

    #41588

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1709A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

16. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-FLEX-rc[Chronos-GFP]

    Plasmid

    #59056

    Purpose

    Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-RFP-miR-Scrint

    Plasmid

    #19825

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    Scrambled miRNA
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only