We narrowed to 5,560 results for: pcas

1. pCAGGS-SARS2-S-FKO (NC-flag)

   Plasmid

   #159363

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Use

   Tags

   Flag and Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SARS2-S-G614 (NC-flag)

   Plasmid

   #156419

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

   Plasmid

   #196197

   Purpose

   Cas9 + gRNA expression to edit G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-Flag-hsDicer (E1564A)

   Plasmid

   #41587

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E1564A

   Promoter

   CMV

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCaSpeR4 mito roGFP2-Grx1

   Plasmid

   #65000

   Purpose

   Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASP-SptP120-3G124-HilA

   Plasmid

   #153323

   Purpose

   Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-3G124 DARPin
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-attBr-Cre-rc-attP

   Plasmid

   #51264

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-Cre-rc-attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT344_pattB-pCA-GFP-pA-FRT5

   Plasmid

   #52550

   Purpose

   for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMMACE DEST CMV SpCas9

    Plasmid

    #206268

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9
    

    Use

    CRISPR; Recombinant baculovirus production

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-PCAF (delta5-53a.a.)

    Plasmid

    #65388

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    PCAF (KAT2B Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    lost 13-159bp of the ORF

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCAGGS-FLAG-BMAL1-S513A

    Plasmid

    #186831

    Purpose

    Expresses FLAG-BMAL1-S513A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-FLAG-BMAL1-S515A

    Plasmid

    #186832

    Purpose

    Expresses FLAG-BMAL1-S515A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 515 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-FLAG-BMAL1-S516A

    Plasmid

    #186833

    Purpose

    Expresses FLAG-BMAL1-S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 516 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-FLAG-BMAL1-S519A

    Plasmid

    #186834

    Purpose

    Expresses FLAG-BMAL1-S519A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 519 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-Flag-S1pr1-R120A

    Plasmid

    #203707

    Purpose

    Overexpression of FLAG-S1PR1-R120A mutant.

    Depositor

    Timothy Hla

    Insert

    Flag-S1PR1-R120A (S1PR1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R120A

    Promoter

    Available since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-Flag-hsDicer (D1320A)

    Plasmid

    #41586

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1320A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCaSper4 cyto roGFP2-Orp1

    Plasmid

    #64998

    Purpose

    Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528-AMOT(1-300)-sortase

    Plasmid

    #166706

    Purpose

    expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT aa 1-300
    

    Use

    Tags

    His-SUMO and Sortase tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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