We narrowed to 2,730 results for: GEM;

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PROM_MpUBE2

Plasmid

#136109

Purpose

PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Adnp

Plasmid

#74340

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Adnp. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

activity-dependent neuroprotective protein (Adnp Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-26 (GB1001)

Plasmid

#68208

Purpose

Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtU6-26 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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PliaI

Plasmid

#55177

Purpose

Bacitracin-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

liaI promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

liaI

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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PliaG

Plasmid

#55174

Purpose

Constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

LiaG promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Adrb2

Plasmid

#105199

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Adrb2 (Adrb2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBW300ParsR-Amp32T

Plasmid

#78652

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pRenilla (GB0056)

Plasmid

#68198

Purpose

Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla coding region

Use

Luciferase and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMB1-B-TelR

Plasmid

#204980

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRBx2

Plasmid

#104476

Purpose

Plasmid carrying FRBx2 with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRBx2 (MTOR Human)

Use

Phagemid

Tags

HA tag

Mutation

includes only the FRB domain

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Emx2

Plasmid

#74346

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

empty spiracles homeobox 2 (Emx2 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMB1-D1-TelR

Plasmid

#204983

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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J23101

Plasmid

#55176

Purpose

Very weak constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

J23101 promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

J23101

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTHsp18.2 (GB0035)

Plasmid

#68186

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only
pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pOpLacI:mini35ss (GB0534)

Plasmid

#68179

Purpose

Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xOpLacI:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBP-pET-RBS

Plasmid

#72981

Purpose

PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

pET RBS derived from pET vectors

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Hox A13b

Plasmid

#36468

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA13b (hoxa11b Zebrafish)

Use

Riboprobe synthesis

Mutation

F232C

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pISH-Dlx3

Plasmid

#74342

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 3 (Dlx3 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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Hox D13a

Plasmid

#36467

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11b (hoxa11b Zebrafish)

Use

Riboprobe synthesis

Mutation

F63I, P75Q and M133I

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pPAtUbq10 (GB0223)

Plasmid

#68174

Purpose

Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq10 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-ORF

Plasmid

#72949

Purpose

Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 0 - ORFs

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Meis2

Plasmid

#74349

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 2 (Meis2 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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