We narrowed to 9,536 results for: pho;

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pSG5 N-HA SADBs shRNA resistant

Plasmid

#67157

Purpose

N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

Promoter

SV40 promoter

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y20,27A

Plasmid

#58465

Purpose

Expresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosines 20 and 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y27A

Plasmid

#58464

Purpose

Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
DIRAS2

Plasmid

#55670

Purpose

expression of mouse DIRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

DIRAS2 (Diras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pMA3411

Plasmid

#46877

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase1 beta 3 (Gucy1b3 Rat)

Use

Retroviral

Mutation

E473K, C541D

Promoter

LTR

Available Since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pSG5-H-Ras_V12-A38

Plasmid

#39513

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 A38 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and D38A

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-E38

Plasmid

#39514

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 E38 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and D38E

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58D,DE)

Plasmid

#25057

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S58D,DE)

Plasmid

#25061

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S44A,DE)

Plasmid

#25054

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 44 to alanine changed leucine 92 …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(T56A,DE)

Plasmid

#25055

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58A,DE)

Plasmid

#25056

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58A,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine changed leucine 92 …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S44D,DE)

Plasmid

#25058

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid changed leuci…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74A,S77A)

Plasmid

#24262

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87A,S90A) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Alanine changed Serine 77 t…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74D,S77D)

Plasmid

#24263

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87D,S90D) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Aspartic Acid changed Serin…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-Flag-Tipin-L195A

Plasmid

#23118

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed Leucine 195 to Alanine

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin-E185A

Plasmid

#23114

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Glutamate 185 to Alanine

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin-E190A

Plasmid

#23115

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Glutamate 190 to Alanine

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pET21b-Tipin-His-L195A

Plasmid

#23123

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Bacterial

Mutation

Changed Leucine 195 to Alanine

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-cGAS C396/7A-HA

Plasmid

#130917

Purpose

Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS C396/7A

Tags

HA

Expression

Mammalian

Mutation

mutated residues 396 and 397 to alanines

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TO-oNGN2

Plasmid

#198397

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2
rtTA3G

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

Promoter

CAG, EF1a, and TRE3G

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Tau(WT)

Plasmid

#187023

Purpose

Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-2xPH-TAPP1 WT

Plasmid

#161990

Purpose

Expresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PH-TAPP1 (PLEKHA1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP-2xFYVE

Plasmid

#140047

Purpose

Expresses GFP-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available Since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10A-HA

Plasmid

#209215

Purpose

Mammalian expression of ATP10A

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10A (ATP10A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only
pAD100-CHIP-myc-His

Plasmid

#236054

Purpose

Mammalian expression of human CHIP with a C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-mCherry

Plasmid

#214274

Purpose

Mammalian expression of LAMP1-mCherry (lysosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

LAMP1-mCherry (Lamp1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge