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Showing: 4251 - 4300 of 4641 results

  16. LRT

    Plasmid
    #214071
    Purpose
    Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR
    Depositor
    Sujatha Jagannathan
    Article
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Insert
    Firefly luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Linker sequence present between firefly luciferas…
    Promoter
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214071-plasmid-map-sequence-id-423951

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  17. pAPV-DsRed2-ER-5

    Plasmid
    #170570
    Purpose
    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170570-plasmid-map-sequence-id-335779

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  22. LiRT

    Plasmid
    #214075
    Purpose
    Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR
    Depositor
    Sujatha Jagannathan
    Article
    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.
    Insert
    Firefly luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Linker sequence present between firefly luciferas…
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214075-plasmid-map-sequence-id-426771

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  31. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177434
    Purpose
    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177434-plasmid-map-sequence-id-348465

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  32. pAPV-DsRed2-FLAG-ER-5

    Plasmid
    #170622
    Purpose
    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170622-plasmid-map-sequence-id-335778

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  35. pICSL50016

    Plasmid
    #50315
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    Use
    Plant expression
    Tags
    Expression
    Mutation
    Codon-optimised for A. thaliana. BsaI/BbsI sites …
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50315-plasmid-map-sequence-id-75072

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  36. Venus-7aa-Cb5-pEGFP-C1

    Plasmid
    #166764
    Purpose
    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5a Rat)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166764-plasmid-map-sequence-id-328853

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  40. BLA_CAT_K136_GPG_LOV2

    Plasmid
    #231575
    Purpose
    This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light
    Depositor
    Dominik Niopek
    Article
    Wolf et al bioRxiv 2024.12.04.626757
    Insert
    CAT_ carrying an AsLOV2 insertion behind K136
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    231575-plasmid-map-sequence-id-471195

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Showing: 4251 - 4300 of 4641 results