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We narrowed to 5,579 results for: PEP

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Showing: 4251 - 4300 of 5579 results

  2. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Available Since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  5. pOCC207

    Plasmid
    #118869
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mCherry
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118869-plasmid-map-sequence-id-232318

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  7. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  9. pOCC230

    Plasmid
    #118871
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mCitrine, cl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118871-plasmid-map-sequence-id-232332

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  10. pOCC69

    Plasmid
    #118874
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mGFP, cleava…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118874-plasmid-map-sequence-id-232330

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  11. pOCC110

    Plasmid
    #118905
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and LPETGg
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118905-plasmid-map-sequence-id-232327

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  12. pOCC219

    Plasmid
    #118908
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
    Tags
    YBBR tag, HIS10, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118908-plasmid-map-sequence-id-232256

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  15. pEG610

    Plasmid
    #40195
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. pTARGEX-CHL

    Plasmid
    #242447
    Purpose
    Targets high levels of recombinant protein to chloroplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    Transit peptide from N. tabacum RbcS
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242447-plasmid-map-sequence-id-490473

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  17. pTARGEX-APO

    Plasmid
    #242446
    Purpose
    Targets high levels of recombinant protein to apoplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    N-terminal fusion with PR1b signal peptide
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242446-plasmid-map-sequence-id-490468

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  18. pTARGEX-ER

    Plasmid
    #242449
    Purpose
    Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    ER-retrieval motif (KDEL) and Signal peptide deri…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242449-plasmid-map-sequence-id-489545

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  19. Methyl-binding domain protein 2 (MBD2) coiled-coil domain

    Plasmid
    #132721
    Purpose
    Bacterial expression vector for methyl-binding domain protein 2 (MBD2) coiled-coil domain for use as competitor peptide in measurements of effective concentrations
    Depositor
    Magnus Kjaergaard
    Article
    Sorensen et al Methods Mol Biol. 2020;2141:505-518. doi
    Insert
    Methyl-binding protein domain 2 coiled-coil domain
    Tags
    N-terminal His-tag. Flanking linkers to match acc…
    Expression
    Bacterial
    Promoter
    T/
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    132721-plasmid-map-sequence-id-260259

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  21. mE-mCherry2A

    Plasmid
    #236248
    Purpose
    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    mCherry2A
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence to mCherry with no stop …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236248-plasmid-map-sequence-id-486041

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  22. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

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  23. SP_C3

    Plasmid
    #228159
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q03178_PIR1_YEAST:0-18
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228159-plasmid-map-sequence-id-451652

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  24. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  32. SARS-CoV spike all5KO mutant

    Plasmid
    #231921
    Purpose
    For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10
    Insert
    spike all5KO (S SARS-CoV)
    Tags
    C9 and CD5 signal peptide
    Expression
    Mammalian
    Mutation
    N1080Q, N1116Q, N1140Q, N1155Q, N1176Q
    Promoter
    CMV
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    231921-plasmid-map-sequence-id-459043

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  36. pLVUT-spC3GFP

    Plasmid
    #225487
    Purpose
    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP
    Depositor
    Robert Gross
    Article
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Secretable and permeable exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251
    Promoter
    Human ubiquitin C
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225487-plasmid-map-sequence-id-444518

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  38. pcDNA3.1-MF3958 Heavy chain-SpyTag003

    Plasmid
    #216303
    Purpose
    Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MF3958 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216303-plasmid-map-sequence-id-433368

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  39. pcDNA3.1-Tras heavy chain-SpyTag003

    Plasmid
    #216293
    Purpose
    Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216293-plasmid-map-sequence-id-433208

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  40. pcDNA3.1-39S Heavy chain-SpyTag003

    Plasmid
    #216296
    Purpose
    Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    39S Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216296-plasmid-map-sequence-id-433365

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  43. pYSD101

    Plasmid
    #212925
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Expression
    Yeast
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    212925-plasmid-map-sequence-id-439532

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  44. pYSD140

    Plasmid
    #212927
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    mRuby2
    Expression
    Yeast
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212927-plasmid-map-sequence-id-421795

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Showing: 4251 - 4300 of 5579 results