We narrowed to 4,338 results for: BFP

1. Fuw-dCas9-dead Tet1CD-P2A-BFP

   Plasmid

   #108246

   Purpose

   Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-dead Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

   Promoter

   Ubc

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]

   Plasmid

   #133327

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211463

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-dead
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   TRE

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

   Plasmid

   #191161

   Purpose

   Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   EGFP.t2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

   Plasmid

   #117134

   Purpose

   All-in-one expression vector for Cas9 and gRNA against CD44

   Depositor

   Kosuke Yusa

   Insert

   gCD44 v2 (Cd44 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #72667

   Purpose

   Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

   Plasmid

   #230935

   Purpose

   gRNA cloning vector encoding BFP and puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Promoter

   hU6

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-UCOE-EF1a-Zim3-dCas9-BFP

   Plasmid

   #188775

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-(BbsI)_CBh-Cas9-T2A-BFP

   Plasmid

   #64323

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-EBFP2

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

    Plasmid

    #236246

    Purpose

    Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    U6 and human Synapsin1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR hEF1A-BFP-P2A-rtTA-WPRE

    Plasmid

    #223288

    Purpose

    A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    BFP-P2A-rtTA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid

    #187952

    Purpose

    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-CasRx-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker, HA-tag, and NLS

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-SFFV-Zim3-dCas9-BFP

    Plasmid

    #188776

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-TRE3G-BCCP-PupE-IRES-BFP

    Plasmid

    #113405

    Purpose

    Express inducible BCCP-PupE in mammalian cell

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    BCCP-PupE-IRES-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

    Plasmid

    #139236

    Purpose

    mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

    Plasmid

    #137993

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. loxP-myctagBFP-STOP-loxP-mCherry-NTR

    Plasmid

    #108456

    Purpose

    This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    loxP-myctagBFP-STOP-loxP-mCherry-NTR
    

    Use

    Cre/Lox

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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