Addgene

We narrowed to 21,856 results for: ESC;

Showing: 1701 - 1720 of 21856 results

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pBAD-jRCaMP1a

Plasmid

#113936

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRCaMP1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mNeonGreen-EC18153

Plasmid

#137075

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al bioRxiv 2023.08.29.555275

Insert

qMaLioffG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-LSSmGFP

Plasmid

#186539

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Use

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pYL322d1/Vn-C

Plasmid

#183154

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-AVP

Plasmid

#184600

Purpose

For expression of a fluorescent sensor for vasopressin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-C124S

Plasmid

#28300

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Phosphatase Inactive Mutant Cys124Ser

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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ReNL-H2B_pcDNA3

Plasmid

#89534

Purpose

Red color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHis17_ParM _2

Plasmid

#78202

Purpose

Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Article

Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

Insert

parM (K33A D63C T174A T175N D224C C287A)

Use

Tags

His6

Expression

Bacterial

Mutation

K33A D63C T174A T175N D224C C287A

Promoter

unknown

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMU-AUTr

Plasmid

#61204

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Use

Tags

Golgi and ER export signal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB9a-GFP

Plasmid

#126112

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23113

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-PGE

Plasmid

#184617

Purpose

For expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1-surrogate-reporter

Plasmid

#65624

Purpose

Fluorescent reporter to monitor the activty of Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attP

Use

Bxb1

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSSmGFP

Plasmid

#186534

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Use

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Cox8x2-CeNL_pcDNA3

Plasmid

#89513

Purpose

Cyan color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

CoxVIII X2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBbB12a-GFP

Plasmid

#126115

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23151

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BRIPO

Plasmid

#215551

Purpose

AAV transfer plasmid for BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Article

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Insert

BRIPO

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn promotor

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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