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pLSSmKate1-C1

Plasmid

#31868

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate1

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Citrine-NLS

Plasmid

#182583

Purpose

Transient expression of Citrine-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

NLS

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMVA

Plasmid

#121149

Purpose

This plamid was used for improving the production of DMAPP (IPP) in E.coli

Depositor

Minjuan Xu

Article

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )

Use

Synthetic Biology

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sen1

Plasmid

#99994

Purpose

Shuttle yeast Sen1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sen1 (SEN1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MIG(F)-CRel

Plasmid

#26984

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

CRel (Rel Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TIM21(1–50)-Citrine

Plasmid

#182584

Purpose

Transient expression of TIM21(1–50)-Citrine in plant cell (Mitochondria)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

TIM21(1–50)

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03R122K/R126K

Plasmid

#28005

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03R122K/R126K

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

R122K, R126K

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pHR Src-TagBFP

Plasmid

#171036

Purpose

TagBFP-tagged Src kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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290 RFP/CFP

Plasmid

#83322

Purpose

fluorescent coding joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA2

Plasmid

#30310

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α2 (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF5

Plasmid

#17984

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF5 (TAF5 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pFUW mEmerald-P2A-NeoR

Plasmid

#239569

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-PuroR

Plasmid

#239566

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGWT35S-OEP7-mCherry

Plasmid

#182587

Purpose

Transient expression of OEP7-mCherry in plant cell (Outer envelope membrane of plastid/chloroplast)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

OEP7

Tags

mCherry

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

Vps50 (Vps50 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-LSSmKate1

Plasmid

#31908

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate1

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pOL2340

Plasmid

#37841

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Ub-M

Tags

mCherry

Expression

Worm

Mutation

R77M

Promoter

nhx-2

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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