Addgene

We narrowed to 13,707 results for: TIM

Showing: 1701 - 1720 of 13707 results

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pEB2-2ndVal mRFP1

Plasmid

#104025

Purpose

Plasmid encoding 2ndVal mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mRFP1

Use

Low copy

Tags

Expression

Bacterial

Mutation

mRFP1 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD468

Plasmid

#66155

Purpose

expression of His-tagged espA

Depositor

James Kaper

Article

Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

Insert

espA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T5

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pFH16

Plasmid

#125801

Purpose

Level0 FnCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

FnCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCVD469

Plasmid

#66156

Purpose

expression of His-tagged espB

Depositor

James Kaper

Article

Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

Insert

espB

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T5

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
pEB2-2ndVal mNept2.5

Plasmid

#104032

Purpose

Plasmid encoding 2ndVal mNeptune2.5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mNeptune2.5

Use

Low copy

Tags

Expression

Bacterial

Mutation

mNeptune2.5 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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BCJJ223

Plasmid

#117509

Purpose

AtMGE1 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pMGE1:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

AtMGE1

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12021

Plasmid

#117511

Purpose

AtICU2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pICU2:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

AtICU2

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP

Plasmid

#48988

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-8XDSR

Plasmid

#48981

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA

Plasmid

#48989

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA tag

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-3XDSR

Plasmid

#48976

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET-21a(+) SurE

Plasmid

#12669

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE (TM1662 T.maritima)

Use

Tags

His

Expression

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pGH912

Plasmid

#230855

Purpose

Ef1a-DivA-BE3-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

DivA-BE3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGH907

Plasmid

#230837

Purpose

Ef1a-MS2-AIDDead-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

MS2-AIDDead

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH518

Plasmid

#230836

Purpose

Ef1a-MS2-AID*-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

MS2-AID*

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH397

Plasmid

#230853

Purpose

Ef1a-AID*-n'Cas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

AID*-n'Cas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH394

Plasmid

#230851

Purpose

Ef1a-rAPOBEC1-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

rAPOBEC1-dCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH389

Plasmid

#230847

Purpose

Ef1a-AID*-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

AID*-dCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH385

Plasmid

#230848

Purpose

Ef1a-dCas9-AID*-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

dCas9-AID*

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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