We narrowed to 1,917 results for: FRT

1. pAAV-nEF-Coff/Fon-bREACHes-EYFP

   Plasmid

   #137147

   Purpose

   Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   Coff/Fon-bREACHes-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

   Plasmid

   #105813

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

   Plasmid

   #105814

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-VCF1 N167A

   Plasmid

   #223019

   Purpose

   Expression of GFP-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

   Depositor

   Niels Mailand

   Article

   Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

   Insert

   VCF1 (VCF1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N167A

   Promoter

   CMV/TetO2

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Strep-HA-VCF1 N167A

   Plasmid

   #223021

   Purpose

   Expression of Strep-HA-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

   Depositor

   Niels Mailand

   Article

   Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

   Insert

   VCF1 (VCF1 Human)
   

   Use

   Tags

   Strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5-TEX264-SIM2_mut-cGFP

   Plasmid

   #220213

   Purpose

   Mammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFP

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

   Promoter

   CMV/TetO

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5'-BirA-FLAG Chtop-exon minimal CMV pCDNA5

   Plasmid

   #212090

   Purpose

   LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Chtop-exon (Chtop Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5'-3xFLAG Camta2-exon minimal CMV pCDNA5

   Plasmid

   #212048

   Purpose

   LR vector for integration of Camta2-exon into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Camta2-exon (Camta2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 5'-3xFLAG Camta2+exon minimal CMV pCDNA5

   Plasmid

   #212049

   Purpose

   LR vector for integration of Camta2+exon(21nt) into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Camta2+exon(21nt) (Camta2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5'-3xFLAG L3mbtl3+exon minimal CMV pCDNA5

    Plasmid

    #212059

    Purpose

    LR vector for integration of L3mbtl3+exon(75nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    L3mbtl3+exon(75nt) (L3mbtl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5'-3xFLAG Chtop+exon_siResist minimal CMV pCDNA5

    Plasmid

    #212083

    Purpose

    LR vector for integration of Chtop+exon(138nt)_siResist into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop+exon(138nt)_siResist (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCCTATATGTCCAAGACCA

    Promoter

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5'-3xFLAG Chtop+exon minimal CMV pCDNA5

    Plasmid

    #212081

    Purpose

    LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop+exon(138nt) (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 5'-3xFLAG Chtop-exon_siResist minimal CMV pCDNA5

    Plasmid

    #212082

    Purpose

    LR vector for integration of Chtop-exon_siResist into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop-exon_siResist (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCCTATATGTCCAAGACCA

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 5'-3xFLAG Med24-exon minimal CMV pCDNA5

    Plasmid

    #212060

    Purpose

    LR vector for integration of Med24-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Med24-exon (Med24 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 5'-3xFLAG Kansl3-exon minimal CMV pCDNA5

    Plasmid

    #212052

    Purpose

    LR vector for integration of Kansl3-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kansl3-exon (Kansl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5'-3xFLAG Kansl3+exon minimal CMV pCDNA5

    Plasmid

    #212053

    Purpose

    LR vector for integration of Kansl3+exon(78nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kansl3+exon(78nt) (Kansl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 5'-3xFLAG Gse1+exon minimal CMV pCDNA5

    Plasmid

    #212057

    Purpose

    LR vector for integration of Gse1+ex(108nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Gse1+ex(108nt) (Gse1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 5'-3xFLAG Kdm1a+exon minimal CMV pCDNA5

    Plasmid

    #212055

    Purpose

    LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a+exon(12nt) (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5'-3xFLAG Med24+exon minimal CMV pCDNA5

    Plasmid

    #212061

    Purpose

    LR vector for integration of Med24+exon(57nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Med24+exon(57nt) (Med24 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 5'-BirA-FLAG Kdm1a-exon minimal CMV pCDNA5

    Plasmid

    #212088

    Purpose

    LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a-exon (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge