We narrowed to 11,503 results for: NSI

1. pCMV-Vamp3-pHluorin

   Plasmid

   #190152

   Purpose

   Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp3 Rat)
   

   Use

   AAV

   Tags

   superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

   Plasmid

   #178528

   Purpose

   Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   rtTA, SspB-mScarlet-I-MYPT (1-169aa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, Tet responsive element

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT_KCNQ3

   Plasmid

   #173163

   Purpose

   To generate Flp-In CHO-KCNQ3 stable cells

   Depositor

   Al George

   Article

   Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

   Insert

   KCNQ3 (KCNQ3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. WDV-Echistatin

   Plasmid

   #226985

   Purpose

   Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

   Depositor

   Wendy Gordon

   Article

   Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

   Inserts

   WDV (repA )
   Echistatin
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. FHL-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129628

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   FHL-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV,&Rhamnose

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-SwitchON-sgVRK1

   Plasmid

   #199638

   Purpose

   Tamoxifen-inducible expression of sgRNA targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lck-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129627

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Lck-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   Lck

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-4eCOL2A1

   Plasmid

   #97211

   Purpose

   For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Vector is designed for cloning and generation of …

   Tags

   Expression

   Mutation

   Promoter

   COL2A1 regulatory elements

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

   Plasmid

   #123194

   Purpose

   binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xnls-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129626

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHub10-His-YFP-PIP2;1

    Plasmid

    #99386

    Purpose

    overexpression and isolation of recombinant At-PIP2;1 protein

    Depositor

    Ning Li

    Article

    Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

    Insert

    PIP2;1 (PIP2A Mustard Weed)
    

    Use

    Tags

    His and YFP

    Expression

    Plant

    Mutation

    Promoter

    2xCaMV35S

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid

    #153442

    Purpose

    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-PI(WT)-Rac1(WT)-mCherry

    Plasmid

    #87550

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-PI(WT)-Rac1(Q61L)-mCherry

    Plasmid

    #87553

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Q61L

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA4 3xFLAG Halo delta 118-180 nsp1

    Plasmid

    #175424

    Purpose

    Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 118-180 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminus of nsp1

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p-AAV2-hSyn-MerMAID6-Citrine

    Plasmid

    #126521

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID6
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSP1-mp53AS-6xMUT

    Plasmid

    #20904

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

    Insert

    p53AS (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    point mutation (Ser/Thr-Ala) of the 6 N-terminal …

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only