We narrowed to 20,033 results for: Nov
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Plasmid#158986PurposeExpresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cellsDepositorInsertGAL4-DrBphP
UseLentiviralTagsMyc tagExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR 2xU6:gRNA, mitfa:Cas9
Plasmid#118844PurposeTargets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-EF1A-CD19.28H.28TM.28.3z
Plasmid#200678PurposeModular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInserteGFP
ExpressionMammalianAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Neo)
Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-rDA3h
Plasmid#208703PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseAAVPromoterhSynAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-H (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
HR-TERT-SV40-GFP
Plasmid#71397PurposeHomologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
TagsSV-40 GFP insertion in TERT promotorPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_cAMPFIRE-L
Plasmid#182283PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP313-pAAV-CMV-SaCas9-DIO-pA
Plasmid#113690PurposeA CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGMCtN-TSC10M1-sspB
Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorPublicationInsertTSC10M1-sspB
Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B2.0
Plasmid#99892PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-GFP
Plasmid#65445PurposeExpression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectorsDepositorInsertSV40-EGFP
UseLentiviralExpressionMammalianPromoterUBQ and SV40Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-P199L
Plasmid#177949PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.DepositorAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY0750 pCAG-NLS-BceINT
Plasmid#179112PurposeBceINT expression vectorDepositorInsertpCAG-NLS-BceINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and NonprofitsCited by