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mAID-BRD4 donor

Plasmid

#140650

Purpose

BRD4 tagging with mAID

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRD4 (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF1a_SPI1_P2A_Hygro_Barcode

Plasmid

#120482

Purpose

Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Publication

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GAL4-DrBphP

Plasmid

#158986

Purpose

Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

Depositor

Liangcai Gu

Publication

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

GAL4-DrBphP

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-EphB4-SV40-GFP (B4G)

Plasmid

#65444

Purpose

Expression of human EPHB4 and GFP in mammalian cells

Depositor

Eduard Batlle

Publication

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB4 (EPHB4 Human)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X

Plasmid

#192151

Purpose

T cell CRISPR AAV-SB vector

Depositor

Sidi Chen

Publication

Ye et al Nat Biotechnol. 2019 Nov;37(11):1302-131

Type

Empty backbone

Use

AAV

Expression

Mammalian

Mutation

NA

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pF4Ag NanoLuc (ATG-42)

Plasmid

#137777

Purpose

Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

Depositor

Lance Encell

Publication

Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NanoLuc (Nluc)

Expression

Bacterial and Mammalian

Mutation

NanoLuc contains 16 amino acid changes compared t…

Promoter

T7 and CMV

Available since

March 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV-EF1A-CD19.28H.28TM.28.3z

Plasmid

#200678

Purpose

Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Publication

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302_HOXB13

Plasmid

#70089

Purpose

Expression of human HOXB13 from CMV promoter, puromycin selection marker

Depositor

William Hahn

Publication

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Publication

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA, mitfa:Cas9

Plasmid

#118844

Purpose

Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Publication

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSyn-GRAB-rDA3h

Plasmid

#208703

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3h

Use

AAV

Promoter

hSyn

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS_cAMPFIRE-H

Plasmid

#182285

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Publication

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cAMPFIRE-H (cAMP sensor)

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-noPS

Plasmid

#177943

Purpose

Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Expression

Mammalian

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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HR-TERT-SV40-GFP

Plasmid

#71397

Purpose

Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Publication

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TERT (TERT Human)

Tags

SV-40 GFP insertion in TERT promotor

Promoter

Endogenous TERT promotor

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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